| Title | Exploration and verification of COVID-19-related hub genes in liver physiological and pathological regeneration |
| Authors | Shi, Jihang Li, Guangya Yuan, Xiandun Wang, Yafei Gong, Ming Li, Chonghui Ge, Xinlan Lu, Shichun |
| Affiliation | Med Sch Chinese Peoples Liberat Army PLA, Beijing, Peoples R China Chinese Peoples Liberat Army Gen Hosp, Fac Hepatopancreatobiliary Surg, Beijing, Peoples R China Inst Hepatobiliary Surg Chinese PLA, Beijing, Peoples R China Peking Univ, Coll Life Sci, Peking Tsinghua Ctr Life Sci, MOE Key Lab Cell Proliferat & Differentiat, Beijing, Peoples R China Peking Univ, Peking Univ Tsinghua Univ Natl Inst Biol Sci Joint, Beijing, Peoples R China Peking Univ Third Hosp, Dept Rheumatol & Immunol, Beijing, Peoples R China |
| Keywords | FAILURE |
| Issue Date | 23-Feb-2023 |
| Publisher | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY |
| Abstract | Objectives An acute injury is often accompanied by tissue regeneration. In this process, epithelial cells show a tendency of cell proliferation under the induction of injury stress, inflammatory factors, and other factors, accompanied by a temporary decline of cellular function. Regulating this regenerative process and avoiding chronic injury is a concern of regenerative medicine. The severe coronavirus disease 2019 (COVID-19) has posed a significant threat to people's health caused by the coronavirus. Acute liver failure (ALF) is a clinical syndrome resulting from rapid liver dysfunction with a fatal outcome. We hope to analyze the two diseases together to find a way for acute failure treatment.Methods COVID-19 dataset (GSE180226) and ALF dataset (GSE38941) were downloaded from the Gene Expression Omnibus (GEO) database, and the "Deseq2 " package and "limma " package were used to identify differentially expressed genes (DEGs). Common DEGs were used for hub genes exploration, Protein-Protein Interaction (PPI) network construction, Gene Ontology (GO) functional enrichment, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. The real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to verify the role of hub genes in liver regeneration during in vitro expansion of liver cells and a CCl4-induced ALF mice model.Results: The common gene analysis of the COVID-19 and ALF databases revealed 15 hub genes from 418 common DEGs. These hub genes, including CDC20, were related to cell proliferation and mitosis regulation, reflecting the consistent tissue regeneration change after the injury. Furthermore, hub genes were verified in vitro expansion of liver cells and in vivo ALF model. On this basis, the potential therapeutic small molecule of ALF was found by targeting the hub gene CDC20.Conclusion We have identified hub genes for epithelial cell regeneration under acute injury conditions and explored a new small molecule Apcin for liver function maintenance and ALF treatment. These findings may provide new approaches and ideas for treating COVID-19 patients with ALF. |
| URI | http://hdl.handle.net/20.500.11897/673040 |
| ISSN | 2296-4185 |
| DOI | 10.3389/fbioe.2023.1135997 |
| Indexed | SCI(E) |
| Appears in Collections: | 生命科学学院 细胞增殖分化调控机理研究教育部重点实验室 第三医院 |
