| Title | Influence of sleep disruption on inflammatory bowel disease and changes in circadian rhythm genes |
| Authors | Wang, Dan Yin, Houqing Wang, Xin Wang, Zequn Han, Mengyuan He, Quanzhao Chen, Jingjing Xian, Haocheng Zhang, Bentuo Wei, Xihua Yang, Baoxue Pan, Yan Li, Jun |
| Affiliation | Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Pharmacol, Beijing 100191, Peoples R China Peking Univ Third Hosp, Dept Gastroenterol, Beijing 100191, Peoples R China Changzhi Med Coll, Dept Pharmacol, Changzhi City 046000, Shanxi Province, Peoples R China Peking Univ, Beijing Key Lab Tumor Syst Biol, Beijing 100191, Peoples R China Beijing Key Lab Helicobacter Pylori Infect & Upper, Beijing, Peoples R China |
| Keywords | DEPRIVATION |
| Issue Date | Oct-2022 |
| Publisher | HELIYON |
| Abstract | According to clinical investigations, sleep disruption (SD) can influence the immune system and cause inflam-matory bowel disease (IBD). However, the detailed effects of sleep on IBD development and progression have not been clarified. Here, we used dextran sulfate sodium (DSS) to induce colitis in mice, and then interfered with SD (day-time 8:00 a.m. to 5:00 p.m.) to explore the influence of sleep on colitis by analyzing colon length, mouse body weight, disease activity index (DAI) score, pathology detection, and infiltration of inflammatory cells with LCA immunohistochemistry analysis. Next, we detected the mRNA levels of circadian genes and related inflam-matory factors, including Bmal1, CLOCK, Cry1, Cry2, Per1, Per2, Timeless, Rev-erb alpha, TNF-alpha, IL-6, and IFN-gamma. Additionally, we conducted a sleep survey in IBD patients and collected colon lesion sites to detect the mRNA levels of those eight circadian genes and three inflammatory factors. We found that SD promoted the body weight decrease, increased inflammation as shown with pathological staining of the DSS animal model, and increased expression of the clock gene Cry2 in DSS-induced colitis mice. In IBD patients with active disease, the mRNA level of circadian genes Bmal1, Cry1, Cry2, and Rev-erb alpha in inflammatory tissues decreased significantly compared with non-inflammatory tissues. |
| URI | http://hdl.handle.net/20.500.11897/672134 |
| DOI | 10.1016/j.heliyon.2022.e11229 |
| Indexed | SCI(E) |
| Appears in Collections: | 第三医院 |
