| Title | Manipulation of Particle/Cell Based on Compressibility in a Divergent Microchannel by Surface Acoustic Wave |
| Authors | Xue, Sen Xu, Qingmei Xu, Zhike Zhang, Xuanhe Zhang, Haixiang Zhang, Xiwen He, Feng Chen, Yiqing Xue, Yu Hao, Pengfei |
| Affiliation | Tsinghua Univ, Dept Engn Mech, Beijing 100084, Peoples R China Peking Univ, Sch Integrated Circuits, Beijing 100871, Peoples R China Nanjing Univ Chinese Med, Sch Med & Holist Integrat Med, Nanjing 210023, Peoples R China |
| Keywords | SEPARATION STIFFNESS CELLS MECHANICS |
| Issue Date | Feb-2023 |
| Publisher | ANALYTICAL CHEMISTRY |
| Abstract | The mechanical properties (compressibility or deformability) of cells are closely related to their death, migration, and differentiation. Accurate separation and manipulation of bioparticles based on these mechanical properties are still a challenging in the field of acoustofluidics. In this work, based on surface acoustic waves (SAW) and divergent microchannels, we developed a new method for separating and detecting particles or cells with different compressibility. The difference in acoustic radiation force (Fr) caused by compressibility are gradually amplified and accumulated by decreasing the flow velocity, and they are finally reflected in the particle migration distance. During the transverse migration process, the alternating dominance of the acoustic radiation force and the Stokes resistance force (Fs) drives the particles to create three typical migration patterns: intermittent migration, compound migration, and near-wall migration. In the present tilted SAW device, a 91% separation success rate of similar to 10 mu m polystyrene (PS) and polydimethylsiloxane (PDMS) particles can be achieved by optimizing the acoustic field input power and the fluid velocity. The application potential of the present divergent microchannel is validated by separating the myelogenous leukemia cell K562 and the natural killer cell NK92 that have similar densities and sizes (similar to 15 mu m) but different compressibility. The results of this work are expected to provide valuable insights into the acoustofluidics separation and detection of the cells that are with different compressibility. |
| URI | http://hdl.handle.net/20.500.11897/672108 |
| ISSN | 0003-2700 |
| DOI | 10.1021/acs.analchem.2c03841 |
| Indexed | SCI(E) |
| Appears in Collections: | 待认领 |
