| Title | Anterograde signaling controls plastid transcription via sigma factors separately from nuclear photosynthesis genes |
| Authors | Hwang, Youra Han, Soeun Yoo, Chan Yul Hong, Liu You, Chenjiang Le, Brandon H. Shi, Hui Zhong, Shangwei Hoecker, Ute Chen, Xuemei Chen, Meng |
| Affiliation | Univ Calif Riverside, Inst Integrat Genome Biol, Dept Bot & Plant Sci, Riverside, CA 92521 USA Capital Normal Univ, Coll Life Sci, Beijing Key Lab Plant Gene Resources & Biotechnol, Beijing 100048, Peoples R China Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China Univ Cologne, Bioctr, Inst Plant Sci, Cologne, Germany Univ Cologne, Bioctr, Cluster Excellence Plant Sci CEPLAS, Cologne, Germany Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA Univ Utah, Sch Biol Sci, Salt Lake City, UT 84112 USA Fudan Univ, Sch Life Sci, Inst Plant Biol, Shanghai 200438, Peoples R China |
| Keywords | LIGHT-DEPENDENT INTERACTION CRYPTOCHROME 1 INTERACTS PHYTOCHROME-A CHLOROPLAST TRANSCRIPTION RNA-POLYMERASE BLUE-LIGHT PIF3 PHOSPHORYLATION ARABIDOPSIS PHOTOMORPHOGENESIS EXPRESSION |
| Issue Date | 2-Dec-2022 |
| Publisher | NATURE COMMUNICATIONS |
| Abstract | Light initiates chloroplast biogenesis in Arabidopsis by eliminating PHYTOCHROME-INTERACTING transcription FACTORs (PIFs), which in turn de-represses nuclear photosynthesis genes, and synchronously, generates a nucleus-to-plastid (anterograde) signal that activates the plastid-encoded bacterial-type RNA polymerase (PEP) to transcribe plastid photosynthesis genes. However, the identity of the anterograde signal remains frustratingly elusive. The main challenge has been the difficulty to distinguish regulators from the plethora of necessary components for plastid transcription and other essential chloroplast functions, such as photosynthesis. Here, we show that the genome-wide induction of nuclear photosynthesis genes is insufficient to activate the PEP. PEP inhibition is imposed redundantly by multiple PIFs and requires PIF3's activator activity. Among the nuclear-encoded components of the PEP holoenzyme, we identify four light-inducible, PIF-repressed sigma factors as anterograde signals. Together, our results elucidate that light-dependent inhibition of PIFs activates plastid photosynthesis genes via sigma factors as anterograde signals in parallel with the induction of nuclear photosynthesis genes. Photoreceptors in the nucleus control plastid transcription by utilizing sigma factors as nucleus-to-plastid signals in parallel with the light regulation of nuclear photosynthesis genes. |
| URI | http://hdl.handle.net/20.500.11897/672029 |
| DOI | 10.1038/s41467-022-35080-0 |
| Indexed | SCI(E) |
| Appears in Collections: | 生命科学学院 |
