| Title | Remote Ischemic Conditioning Mediates Cardio-protection After Myocardial Ischemia/Reperfusion Injury by Reducing 4-HNE Levels and Regulating Autophagy via the ALDH2/SIRT3/HIF1 alpha Signaling Pathway |
| Authors | Gao, Rifeng Lv, Chunyu Qu, Yanan Yang, Hen Hao, Chuangze Sun, Xiaolei Hu, Xiaosheng Yang, Yiqing Tang, Yanhua |
| Affiliation | Nanchang Univ, Affiliated Hosp 2, Dept Cardiovasc Surg, Nanchang, Peoples R China Fudan Univ, Shanghai Peoples Hosp 5, Dept Cardiol, Shanghai, Peoples R China Shenzhen Peking Univ, Hong Kong Univ, Biomed Res Inst, Shenzhen Key Lab Neuronal Struct Biol,Sci & Techno, Shenzhen 518036, Peoples R China Fudan Univ, Zhongshan Hosp, Shanghai Inst Cardiovasc Dis, Dept Cardiol, Shanghai 20032, Peoples R China Zhejiang Univ, Affiliated Hosp 1, Hangzhou, Peoples R China |
| Keywords | DEHYDROGENASE 2 ALDEHYDE HEART ALDH2 TRANSDUCTION SURGERY DAMAGE |
| Issue Date | Feb-2023 |
| Publisher | JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH |
| Abstract | Remote ischemic conditioning (RIC) can be effectively applied for cardio-protection. Here, to clarify whether RIC exerts myocardial protection via aldehyde dehydrogenase 2 (ALDH2), we established a myocardial ischemia/reperfusion (I/R) model in C57BL/6 and ALDH2 knockout (ALDH2-KO) mice and treated them with RIC. Echocardiography and single-cell contraction experiments showed that RIC significantly improved myocardial function and alleviated I/R injury in C57BL/6 mice but did not exhibit its cardioprotective effects in ALDH2-KO mice. TUNEL, Evan's blue/triphenyl tetrazolium chloride, and reactive oxygen species (ROS) assays showed that RIC's effect on reducing myocardial cell apoptosis, myocardial infarction area, and ROS levels was insignificant in ALDH2-KO mice. Our results showed that RIC could increase ALDH2 protein levels, activate sirtuin 3 (SIRT3)/hypoxia-inducible factor 1-alpha (HIF1 alpha), inhibit autophagy, and exert myocardial protection. This study revealed that RIC could exert myocardial protection via the ALDH2/SIRT3/HIF1 alpha signaling pathway by reducing 4-HNE secretion. |
| URI | http://hdl.handle.net/20.500.11897/671154 |
| ISSN | 1937-5387 |
| DOI | 10.1007/s12265-023-10355-z |
| Indexed | SCI(E) |
| Appears in Collections: | 化学生物学与生物技术学院 |
