| Title | The composition and function profile of the gut microbiota of patients with primary Sjogren's syndrome |
| Authors | Wang, Fang Zhufeng, Yunzhi Chen, Zhe Xu, Jun Cheng, Yongjing |
| Affiliation | Beijing Hosp, Natl Ctr Gerontol, Dept Rheumatol & Immunol, Beijing, Peoples R China Peking Univ, Dept Rheumatol, Peoples Hosp, Beijing, Peoples R China Peking Univ, Dept Gastroenterol, Peoples Hosp, Beijing, Peoples R China Peking Univ, Clin Ctr Immune Mediated Digest Dis, Peoples Hosp, Beijing, Peoples R China |
| Keywords | REGULATORY T-CELLS INTERLEUKIN-17 |
| Issue Date | Jan-2023 |
| Publisher | CLINICAL RHEUMATOLOGY |
| Abstract | Objectives This healthy volunteer control-based study was conducted to explore alterations of compositions and function of gut microbiota in Chinese pSS patients. Method The high-throughput Illumina Miseq sequencing method, targeting the V3-V4 region of the 16S ribosomal RNA (rRNA) gene, was used to compare the microbiota communities between 30 pSS patients and 30 age-matched healthy volunteers. The intestinal dysbiosis of pSS patients was evaluated and its correlation with some disease phenotypes was analyzed. Furthermore, we performed the amino acid sequence alignment analysis to illustrate the molecular mimicry patterns of new microbial peptides. Results Compared with that in healthy controls, the composition and function of the gut microbiota significantly differed in pSS patients. Certain genera and species, including genera: Escherichia-Shigella, Sardovia, Veillonella, Insteinimonas, and Lactobacillales; species: Escherichia coli, Lactobacillus phage Sal3, Lactobacillus reuteri, Lactobacillus gasseri, Streptococcus lutetiensis, Streptococcus mutans, Scardovia wiggsiae, and Fusobacterrium ulcerans were found to be enriched in the feces of pSS patients, while butyrate-producing bacteria were less abundant in pSS patients. Certain genera (including Lactobacillales) and species (including Lactobacillus gasseri) were associated with disease severity and therapy resistance parameters. Autoantigen epitopes of "WPSALPT, NPARSFG, MNPARSFG, and AFGLAIGT " from aquaporin-5 were aligned perfectly with one enriched microbiota of patients with pSS, namely Escherichia coli. Conclusions The composition and function of the gut microbiota significantly differed in pSS patients compared with that in healthy controls. Our study would facilitate the possible research on the role of gut microbiota in the pathogenesis of pSS. |
| URI | http://hdl.handle.net/20.500.11897/668168 |
| ISSN | 0770-3198 |
| DOI | 10.1007/s10067-022-06451-1 |
| Indexed | SCI(E) |
| Appears in Collections: | 人民医院 |
