| Title | MALDI-TOF-MS for rapid screening analysis of M-protein in serum |
| Authors | Li, Jie Xu, Anping Xie, Weijie Li, Bowen Yan, Cunliang Xia, Yong Liang, Chao Ji, Ling |
| Affiliation | Peking Univ, Dept Lab Med, Shenzhen Hosp, Shenzhen, Peoples R China Southern Univ Sci & Technol, Sch Life Sci, Dept Biol, Shenzhen, Peoples R China Hong Kong Baptist Univ, Inst Integrated Bioinfomedicine & Translat Sci IBT, Sch Chinese Med, Hong Kong, Peoples R China Beijing Inst Life, Natl Ctr Prot Sci Beijing, State Key Lab Prote, Beijing, Peoples R China |
| Keywords | MINIMAL RESIDUAL DISEASE MONOCLONAL IMMUNOGLOBULINS MASS-SPECTROMETRY ANTIBODY ELECTROPHORESIS IMMUNOFIXATION PEPTIDES |
| Issue Date | 15-Dec-2022 |
| Publisher | FRONTIERS IN ONCOLOGY |
| Abstract | Monoclonal immunoglobin (M-protein) is a serum biomarker for the diagnosis of plasma cell dyscrasias. Despite limitation of analytical sensitivity and resolution, serum protein electrophoresis and immunofixation electrophoresis are still the front-line tests for the detection of M-proteins. Herein, we developed a MALDI-TOF Mass spectrometry-based method for the screening test of M-proteins in human serum. Based on the unique mass signature of different immunoglobin isotypes, M-Proteins could be rapidly identified and typed. The method demonstrated with high analytical performance and throughput, rapid and simple, which could be a new choice for the diagnosis of plasma cell dyscrasias. |
| URI | http://hdl.handle.net/20.500.11897/668048 |
| ISSN | 2234-943X |
| DOI | 10.3389/fonc.2022.1073479 |
| Indexed | SCI(E) |
| Appears in Collections: | 深圳医院 |
