| Title | A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments |
| Authors | Qian,Tongrui Wang,Huan Wang,Peng Geng,Lan Mei,Long Osakada,Takuya Wang,Lei Tang,Yan Kania,Alan Grinevich,Valery Stoop,Ron Lin,Dayu Luo,Minmin Li,Yulong |
| Affiliation | State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China PKU-IDG/McGovern Institute for Brain Research, Beijing, China Medical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China Neuroscience Institute, Department of Psychiatry, New York University Grossman School of Medicine, New York, NY, United States Peking University&ndash,Tsinghua University&ndash,National Institute of Biological Sciences Joint Graduate Program, Peking University, Beijing, China Center for Psychiatric Neuroscience, Department of Psychiatry, Lausanne University Hospital Center (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany National Institute of Biological Sciences (NIBS), Beijing, China Chinese Institute for Brain Research, Beijing, China Tsinghua Institute of Multidisciplinary Biomedical Research (TIMBR), Tsinghua University, Beijing, China Peking&ndash,Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China National Biomedical Imaging Center, Peking University, Beijing, China |
| Keywords | Brain Calcium - Mammals - Peptides |
| Issue Date | 2023 |
| Publisher | Nature Biotechnology |
| Abstract | Oxytocin (OT), a peptide hormone and neuromodulator, is involved in diverse physiological and pathophysiological processes in the central nervous system and the periphery. However, the regulation and functional sequences of spatial OT release in the brain remain poorly understood. We describe a genetically encoded G-protein-coupled receptor activation-based (GRAB) OT sensor called GRABOT1.0. In contrast to previous methods, GRABOT1.0 enables imaging of OT release ex vivo and in vivo with suitable sensitivity, specificity and spatiotemporal resolution. Using this sensor, we visualize stimulation-induced OT release from specific neuronal compartments in mouse brain slices and discover that N-type calcium channels predominantly mediate axonal OT release, whereas L-type calcium channels mediate somatodendritic OT release. We identify differences in the fusion machinery of OT release for axon terminals versus somata and dendrites. Finally, we measure OT dynamics in various brain regions in mice during male courtship behavior. Thus, GRABOT1.0 provides insights into the role of compartmental OT release in physiological and behavioral functions. © 2023, The Author(s), under exclusive licence to Springer Nature America, Inc. |
| URI | http://hdl.handle.net/20.500.11897/666882 |
| ISSN | 1087-0156 |
| DOI | 10.1038/s41587-022-01561-2 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 生命科学学院 膜生物学国家重点实验室 前沿交叉学科研究院 |
