| Title | Interbacterial Chemical Communication-Triggered Nascent Proteomics |
| Authors | Liu, Weibing Tang, Qi Meng, Liying Hu, Shufan Sun, De-en Li, Shan Dai, Peng Chen, Xing |
| Affiliation | Peking Univ, Coll Chem & Mol Engn, Peking Tsinghua Ctr Life Sci, Synthet & Funct Biomol Ctr,Beijing Natl Lab Mol Sc, Beijing 100871, Peoples R China Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Minist Educ, Beijing 100871, Peoples R China Shandong Univ, Qilu Hosp Qingdao, Cheeloo Coll Med, Dept Med Expt Ctr, Qingdao 266035, Peoples R China Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Hubei, Peoples R China Huazhong Agr Univ, Coll Biomed & Hlth, Wuhan, Hubei, Peoples R China |
| Keywords | AMINO-ACID PRECURSORS PROTEINS IDENTIFICATION LUXR |
| Issue Date | Dec-2022 |
| Publisher | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION |
| Abstract | Metabolic labeling with clickable noncanonical amino acids has enabled nascent proteome profiling, which can be performed in a cell-type-specific manner. However, nascent proteomics in an intercellular communication-dependent manner remains challenging. Here we develop communication-activated profiling of protein expression (CAPPEX), which integrates the LuxI/LuxR quorum sensing circuit with the cell-type-specific nascent proteomics method to enable selective click-labeling of newly synthesized proteins in a specific bacterium upon receiving chemical signals from another reporter bacterium. CAPPEX reveals that E. coli competes with Salmonella for tryptophan as the precursor for indole, and the resulting indole suppressed the expression of virulence factors in Salmonella. This tryptophan-indole axis confers attenuation of Salmonella invasion in host cells and living mice. The CAPPEX strategy should be widely applicable for investigating various interbacterial communication processes. |
| URI | http://hdl.handle.net/20.500.11897/662017 |
| ISSN | 1433-7851 |
| DOI | 10.1002/anie.202214010 |
| Indexed | SCI(E) |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ 生物有机与分子工程教育部重点实验室 |
