| Title | Electrochemical immunosensor for ultrasensitive detection of human papillomaviruse type 16 L1 protein based on Ag@AuNPs-GO/SPA |
| Authors | Wang, Aiping Zhou, Yiting Chen, Yumei Zhou, Jingming You, Xiaojuan Liu, Hongliang Liu, Yankai Ding, Peiyang Qi, Yanhua Liang, Chao Zhu, Xifang Zhang, Ying Liu, Enping Zhang, Gaiping |
| Affiliation | Zhengzhou Univ, Sch Life Sci, Zhengzhou 450000, Peoples R China Longhu Lab, Zhengzhou 451100, Peoples R China Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China |
| Keywords | CAPSID PROTEIN AU NANOCOMPOSITES NANOPARTICLES HYDROQUINONE ELECTRODES CATECHOL SAMPLES DNA |
| Issue Date | 1-Jan-2023 |
| Publisher | ANALYTICAL BIOCHEMISTRY |
| Abstract | Human papillomaviruse type 16 (HPV16) is a high-risk serotype. As the main protective antigen protein, L1 protein is also the target protein for diagnosis. A simple label free electrochemical immunosensor (ECIS) was fabricated for ultrasensitive detection of HPV16 L1 protein in this work. Quasi-spherical Ag@Au core-shell nanoparticles on graphene oxide (Ag@AuNPs-GO) was developed as current response amplifier and character-ized by UV-Vis Spectroscopy, Transmission Electron Microscopy and energy dispersive X-ray spectroscopy. Staphylococcal protein A was decorated on the modified electrode and utilized to immobilized the Fc portion of the monoclonal antibody specific for HPV16 L1 protein. Cyclic Voltammetry, Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy were used to verify the electrochemical performance and interfacial kinetic property. The increased concentration of HPV16 L1 protein led to slow electron transport and linearly decreased differential pulse voltammetry peak current with a detection limit of 0.002 ng mL-1 and a wide linear relationship in the range of 0.005-400 ng mL-1at a regression coefficient (R2) of 0.9948. Furthermore, this ECIS demonstrated acceptable accuracy with good reproducibility, stability and selectivity, suggesting a promising immunological strategy for HPV typing and early screening. |
| URI | http://hdl.handle.net/20.500.11897/660892 |
| ISSN | 0003-2697 |
| DOI | 10.1016/j.ab.2022.114953 |
| Indexed | EI SCI(E) |
| Appears in Collections: | 现代农学院 |
