| Title | Convergence of MCR-8.2 and Chromosome-Mediated Resistance to Colistin and Tigecycline in an NDM-5-Producing ST656 Klebsiella pneumoniae Isolate From a Lung Transplant Patient in China |
| Authors | Zhao, Jiankang Li, Ziyao Zhang, Yulin Liu, Xinmeng Lu, Binghuai Cao, Bin |
| Affiliation | China Japan Friendship Hosp, Lab Clin Microbiol & Infect Dis, Dept Pulm & Crit Care Med,Natl Clin Res Ctr Resp, Natl Ctr Resp Med,Ctr Resp Med, Beijing, Peoples R China Chinese Acad Med Sci & Peking Union Med Coll, Inst Resp Med, Beijing, Peoples R China Peking Univ, Tsinghua Univ, Joint Ctr Life Sci, Beijing, Peoples R China Capital Med Univ, Dept Resp Med, Beijing, Peoples R China |
| Keywords | ESCHERICHIA-COLI BETA-LACTAMASE MECHANISMS SUSCEPTIBILITY PLASMIDS RAMA |
| Issue Date | 11-Jul-2022 |
| Publisher | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY |
| Abstract | We characterized the first NDM- 5 and MCR- 8.2 co- harboring ST656 Klebsiella pneumoniae clinical isolate, combining with chromosomal gene-mediated resistance to colistin and tigecycline. The K. pneumoniae KP32558 was isolated from the bronchoalveolar lavage fluid from a lung transplant patient. Complete genome sequences were obtained through Illumina HiSeq sequencing and nanopore sequencing. The acquired resistance genes and mutations in chromosome-encoded genes associated with colistin and tigecycline resistance were analyzed. Comparative genomic analysis was conducted between mcr-8.2-carrying plasmids. The K. pneumoniae KP32558 was identified as a pan-drug resistant bacteria, belonging to ST656, and harbored plasmid-encoded blaNDM- 5 and mcr-8.2 genes. The blaNDM- 5 gene was located on an IncX3 type plasmid. The mcr-8.2 gene was located on a conjugative plasmid pKP32558-2-mcr8, which had a common ancestor with another two mcr-8.2carrying plasmids pMCR8_020135 and pMCR8_095845. The MIC of KP32558 for colistin was 256 mg/L. The mcr-8.2 gene and mutations in the two- component system, pmrA and crrB, and the regulator mgrB, had a synergistic effect on the highlevel colistin resistance. The truncation in the acrR gene, related to tigecycline resistance, was also identified. K. pneumoniae has evolved a variety of complex resistance mechanisms to the last-resort antimicrobials, close surveillance is urgently needed to monitor the prevalence of this clone. |
| URI | http://hdl.handle.net/20.500.11897/658530 |
| ISSN | 2235-2988 |
| DOI | 10.3389/fcimb.2022.922031 |
| Indexed | SCI(E) |
| Appears in Collections: | 生命科学学院 |
