| Title | OsMADS58 Stabilizes Gene Regulatory Circuits during Rice Stamen Development |
| Authors | Shen, Liping Tian, Feng Cheng, Zhukuan Zhao, Qiang Feng, Qi Zhao, Yan Han, Bin Fang, Yuhan Lin, Yanan Chen, Rui Wang, Donghui Sun, Wenfeng Sun, Jiaqi Zeng, Hongyun Yao, Nan Gao, Ge Luo, Jingchu Xu, Zhihong Bai, Shunong |
| Affiliation | Peking Univ, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China Peking Univ, Biomed Pioneering Innovat Ctr Biop, Beijing Adv Innovat Ctr Genom ICG, Ctr Bioinformat CBI, Beijing 100871, Peoples R China Peking Univ, Ctr Bioinformat, Beijing 100871, Peoples R China Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Yangzhou 225009, Jiangsu, Peoples R China Chinese Acad Sci, Natl Ctr Gene Res, State Key Lab Plant Mol Genet, CAS Ctr Excellence Mol Plant Sci,Inst Plant Physi, Shanghai 200233, Peoples R China Novogene, Beijing 100015, Peoples R China Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510260, Peoples R China Peking Univ, Ctr Quantitat Biol, Beijing 100871, Peoples R China |
| Keywords | SYNAPTONEMAL COMPLEX MOLECULAR ANALYSIS FLOWER DEVELOPMENT ARABIDOPSIS REVEALS ENCODES SPOROGENESIS INITIATION ALIGNMENT MERISTEM |
| Issue Date | Nov-2022 |
| Publisher | PLANTS-BASEL |
| Abstract | Rice (Oryza sativa) OsMADS58 is a C-class MADS box protein, and characterization of a transposon insertion mutant osmads58 suggested that OsMADS58 plays a role in stamen development. However, as no null mutation has been obtained, its role has remained unclear. Here, we report that the CRISPR knockout mutant osmads58 exhibits complex altered phenotypes, including anomalous diploid germ cells, aberrant meiosis, and delayed tapetum degeneration. This CRISPR mutant line exhibited stronger changes in expression of OsMADS58 target genes compared with the osmads58 dSpm (transposon insertion) line, along with changes in multiple pathways related to early stamen development. Notably, transcriptional regulatory circuits in young panicles covering the stamen at stages 4-6 were substantially altered in the CRISPR line compared to the dSpm line. These findings strongly suggest that the pleiotropic effects of OsMADS58 on stamen development derive from a potential role in stabilizing gene regulatory circuits during early stamen development. Thus, this work opens new avenues for viewing and deciphering the regulatory mechanisms of early stamen development from a network perspective. |
| URI | http://hdl.handle.net/20.500.11897/658369 |
| DOI | 10.3390/plants11212899 |
| Indexed | SCI(E) |
| Appears in Collections: | 其他实验室 生命科学学院 |
