| Title | Anaerobic ammonium oxidation coupled to arsenate reduction, a novel biogeochemical process observed in arsenic-contaminated paddy soil |
| Authors | Zhang, Miaomiao Kolton, Max Haggblom, Max M. Sun, Xiaoxu Yu, Ke He, Bin Yuan, Zaijian Dong, Yiran Su, Xianfa Chen, Zhenyu Li, Hui Xiao, Tangfu Xiao, Enzong Sun, Weimin |
| Affiliation | Guangdong Acad Sci, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangdong Key Lab Integrated Agroenvironm Pollut, Inst Ecoenvironm & Soil Sci, Guangzhou 510650, Peoples R China Guangzhou Univ, Sch Environm Sci & Engn, Key Lab Water Qual & Conservat Pearl River Delta, Minist Educ, Guangzhou 510006, Peoples R China Ben Gurion Univ Negev, French Associates Inst Agr & Biotechnol Drylands, Jacob Blaustein Inst Desert Res, Sede Boqer Campus, IL-8499000 Beer Sheva, Israel Rutgers State Univ, Dept Biochem & Microbiol, New Brunswick, NJ 08901 USA Peking Univ, Sch Environm & Energy, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China China Univ Geosci Wuhan, Sch Environm Studies, Wuhan 430074, Peoples R China Henan Normal Univ, Sch Environm, Key Lab Yellow River & Huai River Water Environm, Minist Educ, Xinxiang 453007, Henan, Peoples R China Jinan Univ, Coll Life Sci & Technol, Dept Ecol, Guangzhou 510632, Peoples R China |
| Keywords | NITROGEN LOSS ANAMMOX BACTERIA IRON REDUCTION WATER DENITRIFICATION CHROMATE HALOTOLERANT BIODIVERSITY MOBILIZATION MANGANESE |
| Issue Date | 15-Oct-2022 |
| Publisher | GEOCHIMICA ET COSMOCHIMICA ACTA |
| Abstract | Anaerobic ammonium oxidation represents an important pathway of N loss, which can be coupled with reduction of nitrite and metal(loid)s (e.g., Fe(III) and Mn(IV)). Similar to Fe(III) and Mn(IV), As(V) is also an active metal(loid) and ammonium oxidation coupled with As(V) reduction is thermodynamically feasible. However, little is known about this poten-tial process. In this study, anaerobic ammonium oxidation coupled with As(V) reduction, designated as Asammox, was observed in cultures inoculated from As-contaminated paddy soil using N-15 isotope tracer analysis. Compared with the treat-ment amended with N-15-urea only, the production of N-15-labeled N-2 (i.e., N-30(2) and N-29(2)) was significantly greater in the treat-ment amended with As(V) and N-15-urea. Furthermore, the abundances of the genes encoding for arsenate reductase (arrA) and hydrazine synthase (hzsB) were significantly higher in the treatment amended with As(V) and N-15-urea than those in the treatment amended with N-15-urea only. In addition, putative Asammox bacteria affiliated with Halomonas, Pelagibacterium, and Chelativorans were identified by DNA-stable isotope probing. Members of Ca. Brocadia were the most dominant Anam-mox bacteria in the soil cultures and may interact with Asammox bacteria in ammonium oxidation, suggesting that the N loss may be attributed to the contribution of Asammox and Anammox in the As-contaminated soil. The observation of Asam-mox, a novel biogeochemical process, and identification of bacteria responsible for this biogeochemical process expands the fundamental understanding of both N and As biogeochemical cycling. In addition, this study provides a proof-of-concept for investigating anaerobic ammonium oxidation coupled with metal(loid)s reduction by combining stable isotope probing and isotope tracer microcosm incubations. (C) 2022 Elsevier Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/654733 |
| ISSN | 0016-7037 |
| DOI | 10.1016/j.gca.2022.08.020 |
| Indexed | SCI(E) |
| Appears in Collections: | 环境与能源学院 |
