| Title | Fluorescence Imaging of Neural Activity, Neurochemical Dynamics, and Drug-Specific Receptor Conformation with Genetically Encoded Sensors |
| Authors | Dong, Chunyang Zheng, Yu Long-Iyer, Kiran Wright, Emily C. Li, Yulong Tian, Lin |
| Affiliation | Univ Calif Davis, Grad Program Biochem Mol Cellular & Dev Biol, Davis, CA 95616 USA Univ Calif Davis, Sch Med, Dept Biochem & Mol Med, Davis, CA 95616 USA Peking Univ, State Key Lab Membrane Biol, Sch Life Sci, Beijing, Peoples R China PKU IDG McGovern Inst Brain Res, Beijing, Peoples R China Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China Univ Calif Davis, Neurosci Grad Program, Davis, CA 95616 USA |
| Keywords | IN-VIVO BRAIN CHEMISTRY CALCIUM VOLTAGE CA2+ INDICATORS PROTEINS EXPRESSION NEURONS FLIES |
| Issue Date | 2022 |
| Publisher | ANNUAL REVIEW OF NEUROSCIENCE |
| Abstract | Recent advances in fluorescence imaging permit large-scale recording of neural activity and dynamics of neurochemical release with unprecedented resolution in behaving animals. Calcium imaging with highly optimized genetically encoded indicators provides a mesoscopic view of neural activity from genetically defined populations at cellular and subcellular resolutions. Rigorously improved voltage sensors and microscopy allow for robust spike imaging of populational neurons in various brain regions. In addition, recent protein engineering efforts in the past few years have led to the development of sensors for neurotransmitters and neuromodulators. Here, we discuss the development and applications of these genetically encoded fluorescent indicators in reporting neural activity in response to various behaviors in different biological systems as well as in drug discovery. We also report a simple model to guide sensor selection and optimization. |
| URI | http://hdl.handle.net/20.500.11897/649293 |
| ISSN | 0147-006X |
| DOI | 10.1146/annurev-neuro-110520-031137 |
| Indexed | SCI(E) |
| Appears in Collections: | 生命科学学院 膜生物学国家重点实验室 |
