| Title | Genetic encoding of epsilon-N-l-lactyllysine for detecting delactylase activity in living cells |
| Authors | Sun, Yanan Chen, Yanchi Xu, Yaxin Zhang, Yuqing Lu, Minghao Li, Manjia Zhou, Liyan Peng, Tao |
| Affiliation | Peking Univ, Guangdong Prov Key Lab Chem Genom, State Key Lab Chem Oncogen, Sch Chem Biol & Biotechnol,Shenzhen Grad Sch, Shenzhen 518055, Peoples R China Shenzhen Bay Lab, Inst Chem Biol, Shenzhen 518132, Peoples R China |
| Keywords | TRANSFER-RNA SYNTHETASE METABOLIC-REGULATION FLUORESCENT-PROBE LYSINE EXPRESSION INHIBITOR EXPANSION LACTATE DESIGN POTENT |
| Issue Date | Jul-2022 |
| Publisher | CHEMICAL COMMUNICATIONS |
| Abstract | Lysine epsilon-N-l-lactylation is a newly discovered post-translational modification. Herein we present the genetic encoding of epsilon-N-l-lactyllysine in bacterial and mammalian cells, allowing the preparation of site-specifically epsilon-N-l-lactylated recombinant proteins and the construction of fluorescent and luminescent probes for detecting delactylases in living cells. Using these probes, we demonstrate sirtuin 1 as a potential delactylase for non-histone proteins. |
| URI | http://hdl.handle.net/20.500.11897/648977 |
| ISSN | 1359-7345 |
| DOI | 10.1039/d2cc02643k |
| Indexed | SCI(E) |
| Appears in Collections: | 化学生物学与生物技术学院 |
