| Title | Hepatocellular cystathionine gamma lyase/hydrogen sulfide attenuates nonalcoholic fatty liver disease by activating farnesoid X receptor |
| Authors | Xu, Wenjing Cui, Changting Cui, Chunmei Chen, Zhenzhen Zhang, Haizeng Cui, Qinghua Xu, Guoheng Fan, Jianglin Han, Yu Tang, Liangjie Targher, Giovanni Byrne, Christopher D. Zheng, Ming-Hua Yang, Liming Cai, Jun Geng, Bin |
| Affiliation | Xian Med Univ, Sch Basic Med Sci, Dept Pathol, Xian, Shaanxi, Peoples R China Chinese Acad Med Sci & Peking Union Med Coll, Hypertens Ctr, Natl Ctr Cardiovasc Dis, State Key Lab Cardiovasc Dis,Fuwai Hosp, Beijing, Peoples R China Peking Univ, Sch Basic Med Sci, Dept Bioinformat Physiol & Pathophysiol, Beijing, Peoples R China Wenzhou Med Univ, Dept Gastrointestinal Surg, Affiliated Hosp 1, Wenzhou, Peoples R China Wenzhou Med Univ, NAFLD Res Ctr, Dept Hepatol, Affiliated Hosp 1, Wenzhou, Peoples R China Key Lab Diag & Treatment Dev Chron Liver Dis Zhej, Wenzhou, Peoples R China Univ & Azienda Osped Univ Integrata Verona, Dept Med, Sect Endocrinol Diabet & Metab, Verona, Italy Univ Hosp Southampton, Southampton Gen Hosp, Southampton Natl Inst Hlth, Southampton, Hants, England Harbin Med Univ Daqing, Dept Pathophysiol, Daqing, Peoples R China |
| Keywords | HYDROGEN-SULFIDE BILE-ACIDS OBETICHOLIC ACID BETA-SYNTHASE FXR LYASE STEATOHEPATITIS TRIGLYCERIDE EXPRESSION GENE |
| Issue Date | Jun-2022 |
| Publisher | HEPATOLOGY |
| Abstract | Background and Aims Hydrogen sulfide (H2S) plays a protective role in NAFLD. However, whether cystathionine gamma lyase (CSE), a dominant H2S generating enzyme in hepatocytes, has a role in the pathogenesis of NAFLD is currently unclear. Approach and Results We showed that CSE protein expression is dramatically downregulated, especially in fibrotic areas, in livers from patients with NAFLD. In high-fat diet (HFD)-induced NAFLD mice or an oleic acid-induced hepatocyte model, the CSE/H2S pathway is also downregulated. To illustrate a regulatory role for CSE in NAFLD, we generated a hepatocyte-specific CSE knockout mouse (CSELKO). Feeding an HFD to CSELKO mice, they showed more hepatic lipid deposition with increased activity of the fatty acid de novo synthesis pathway, increased hepatic insulin resistance, and higher hepatic gluconeogenic ability compared to CSELoxp control mice. By contrast, H2S donor treatment attenuated these phenotypes. Furthermore, the protection conferred by H2S was blocked by farnesoid X receptor (FXR) knockdown. Consistently, serum deoxycholic acid and lithocholic acid (FXR antagonists) were increased, and tauro-beta-muricholic acid (FXR activation elevated) was reduced in CSELKO. CSE/H2S promoted a post-translation modification (sulfhydration) of FXR at Cys138/141 sites, thereby enhancing its activity to modulate expression of target genes related to lipid and glucose metabolism, inflammation, and fibrosis. Sulfhydration proteomics in patients' livers supported the CSE/H2S modulation noted in the CSELKO mice. Conclusions FXR sulfhydration is a post-translational modification affected by hepatic endogenous CSE/H2S that may promote FXR activity and attenuate NAFLD. Hepatic CSE deficiency promotes development of nonalcoholic steatohepatitis. The interaction between H2S and FXR may be amenable to therapeutic drug treatment in NAFLD. |
| URI | http://hdl.handle.net/20.500.11897/648496 |
| ISSN | 0270-9139 |
| DOI | 10.1002/hep.32577 |
| Indexed | SCI(E) |
| Appears in Collections: | 基础医学院 |
