| Title | Proteomic Analysis of Human Milk Reveals Nutritional and Immune Benefits in the Colostrum from Mothers with COVID-19 |
| Authors | Guo, Juanjuan Tan, Minjie Zhu, Jing Tian, Ye Liu, Huanyu Luo, Fan Wang, Jianbin Huang, Yanyi Zhang, Yuanzhen Yang, Yuexin Wang, Guanbo |
| Affiliation | Wuhan Univ, Zhongnan Hosp, Hubei Clin Res Ctr Prenatal Diag & Birth Hlth, Dept Obstet & Gynaecol, Wuhan 430071, Peoples R China Shenzhen Bay Lab, Inst Cell Anal, Shenzhen 518132, Peoples R China Beijing Acad Sci & Technol, Inst Biotechnol & Hlth, Beijing 100089, Peoples R China Wuhan Univ, Inst Med Virol, Sch Basic Med Sci, State Key Lab Virol, Wuhan 430071, Peoples R China Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China Peking Univ, Biomed Pioneering Innovat Ctr, Beijing 100871, Peoples R China Hubei Prov Key Lab Developmentally Originated Dis, Wuhan 430071, Peoples R China Chinese Ctr Dis Control & Prevent, Natl Inst Nutr & Hlth, Beijing 100050, Peoples R China |
| Keywords | BIOACTIVE PROTEINS BREAST-MILK TRACT SARS-COV-2 INFECTION ANTIBODY INFANTS SYSTEM HEALTH |
| Issue Date | Jun-2022 |
| Publisher | NUTRIENTS |
| Abstract | Despite the well-known benefits of breastfeeding and the World Health Organization's breastfeeding recommendations for COVID-19 infected mothers, whether these mothers should be encouraged to breastfeed is under debate due to concern about the risk of virus transmission and lack of evidence of breastmilk's protective effects against the virus. Here, we provide a molecular basis for the breastfeeding recommendation through mass spectrometry (MS)-based proteomics and glycosylation analysis of immune-related proteins in both colostrum and mature breastmilk collected from COVID-19 patients and healthy donors. The total protein amounts in the COVID-19 colostrum group were significantly higher than in the control group. While casein proteins in COVID-19 colostrum exhibited significantly lower abundances, immune-related proteins, especially whey proteins with antiviral properties against SARS-CoV-2, were upregulated. These proteins were detected with unique site-specific glycan structures and improved glycosylation diversity that are beneficial for recognizing epitopes and blocking viral entry. Such adaptive differences in milk from COVID-19 mothers tended to fade in mature milk from the same mothers one month postpartum. These results suggest that feeding infants colostrum from COVID-19 mothers confers both nutritional and immune benefits, and provide molecular-level insights that aid breastmilk feeding decisions in cases of active infection. |
| URI | http://hdl.handle.net/20.500.11897/648465 |
| DOI | 10.3390/nu14122513 |
| Indexed | SCI(E) |
| Appears in Collections: | 待认领 |
