| Title | Fast, Reusable, Cell Uniformly Distributed Membrane Filtration Device for Separation of Circulating Tumor Cells |
| Authors | Han, Jintao Lu, Chunyang Shen, Mengzhu Sun, Xiaoyi Mo, Xiaodong Yang, Gen |
| Affiliation | Peking Univ, Sch Phys, State Key Lab Nucl Phys & Technol, Beijing 100871, Peoples R China Peking Univ, Inst Hematol, Beijing Key Lab Hematopoiet Stem Cell Transplanta, Peoples Hosp, Beijing 100044, Peoples R China Univ Chinese Acad Sci, Wenzhou Inst, Wenzhou 352001, Peoples R China |
| Keywords | CANCER-CELLS MICROCHIP BLOOD |
| Issue Date | 21-Jun-2022 |
| Publisher | ACS OMEGA |
| Abstract | Isolation of circulating tumor cells (CTCs) is of great significance for the diagnosis, prognosis, and treatment of metastatic cancer. Among CTC capture methods independent of antibodies, membrane filtration-based methods have the advantages of simplicity, rapidity, and high throughput but usually have problems such as dogging, high pressure drop, and impaired cell viability. In this study, we designed and tested a reusable device that used horizontal rotor and fluid-assisted separation to capture CTCs by centrifugal membrane filtration, achieving simple, fast, highly efficient, and viable cell capture on traditional centrifuge. The average capture efficiency was 95.8% for different types of cancer cells with >90% survival, and the removal of white blood cells can reach 99.72% under four times cleaning of the membrane after filtration. A further clinic demo was performed using the device to detect residual leukemic cells in patients; the results showed a 10-fold enrichment of the leukemic cells in peripheral blood samples. Taken together, the simple, robust, and efficient CTC capture device may have the potential for clinic routine detection and analysis of circulating tumor cells. |
| URI | http://hdl.handle.net/20.500.11897/648271 |
| ISSN | 2470-1343 |
| DOI | 10.1021/acsomega.2c01153 |
| Indexed | SCI(E) |
| Appears in Collections: | 物理学院 核物理与核技术国家重点实验室 医学部待认领 |
