Title | Alteration of tight junctions during botulinum toxin type A-inhibited salivary secretion |
Authors | Mao, Qian-Ying Xie, Shang Wu, Li-Ling Xiang, Ruo-Lan Cai, Zhi-Gang |
Affiliation | Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, 22 Zhong Guan Cun South St, Beijing 100081, Peoples R China Peking Univ, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci, Minist Educ,Sch Basic Med Sci, 38 Xueyuan Rd, Beijing 100191, Peoples R China |
Keywords | PROTEINS BARRIER PERMEABILITY SIALORRHEA DISRUPTION MODULATION CLAUDIN-3 ION |
Issue Date | Jun-2022 |
Publisher | ORAL DISEASES |
Abstract | Objectives Tight junctions (TJs) are involved in the regulation of salivary secretion via paracellular pathway. Botulinum toxin type A (BTXA) is widely used for the treatment of hypersecretion diseases such as sialorrhea. This study aimed to investigate the role of TJs in BTXA-inhibited secretion of the submandibular gland (SMG). Materials and Methods BTXA was injected into the SMGs of rats, and the same amount of saline was injected as a control. Western blot, real-time PCR, and immunofluorescence staining were used to detect the expression and distribution of TJ proteins. Paracellular permeability was evaluated using the transepithelial electrical resistance (TER) measurements and fluorescent tracer detection in BTXA-stimulated SMG-C6 cells. Results BTXA injection into the SMGs of rats led to increased expression of claudin (Cldn) -1 and Cldn3. Immunofluorescence staining showed no significant changes in the distribution of TJ proteins. In vitro, BTXA increased the TER values and significantly reduced the permeability of fluorescent tracer, suggesting that BTXA decreased the paracellular permeability. The expression levels of Cldn1, Cldn3, and Cldn4 were upregulated after BTXA treatment. Conclusion The expression of TJ proteins changed in both animal models and SMG-C6 cells after BTXA treatment, which may contribute to the inhibition of salivary secretion. |
URI | http://hdl.handle.net/20.500.11897/647445 |
ISSN | 1354-523X |
DOI | 10.1111/odi.14223 |
Indexed | SCI(E) |
Appears in Collections: | 口腔医院 分子心血管学教育部重点实验室 |