| Title | Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells |
| Authors | Wang, Rui Yang, Dongyan Tian, Tian An, Yuhao Wan, Chuan Chang, Qi Liang, Mingchan Hou, Zhanfeng Wang, Ying Zhang, Liang Li, Zigang |
| Affiliation | Pingshan Translat Med Ctr, Shenzhen Bay Lab, Shenzhen 518118, PR, Peoples R China Zhongkai Univ Agr & Engn, Innovat Inst Plant Hlth, Coll Chem & Chem Engn, Guangzhou 510225, Peoples R China Shenzhen Res Inst City Univ Hong Kong, Biotech & Hlth Ctr, Key Lab Biochip Technol, Shenzhen 518057, Peoples R China City Univ Hong Kong, Dept Biomed Sci, Coll Vet Med & Life Sci, Kowloon, Hong Kong 999077, Peoples R China Peking Univ, State Key Lab Chem Oncogen, Guangdong Prov Key Lab Chem Genom, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China |
| Keywords | CYCLOADDITION MOLECULES |
| Issue Date | 15-Mar-2022 |
| Publisher | ANALYTICAL CHEMISTRY |
| Abstract | Despite being a low-abundance amino acid, cysteine plays an essential role in regulating protein function and serves as a satisfactory target of post-translational modifications and drug developments. To comprehensively assess reactive-cysteine-containing proteins, the development of chemical proteomic probes to label cysteine residues in human cells is an important objective. Cysteine modification using sulfonium-based probes is a novel method to identify reactive cysteine residues in proteins. Herein, we reported a set of "cysteine-reactive sulfonium-based (C-Sul)" probes to label the reactive cysteine sites in cellular proteins. Notably, water-soluble C-Sul probes have a significantly enhanced stability and cellular uptakes, displaying a high specificity toward reactive cysteines and compatibility with quantitative proteomic profiling. In comparison to the conventional iodoacetamide-based probe, C-Sul particularly has no inhibitory effects on cell viability, enabling its application in proteomic profiling of reactive cysteine residues under biorelevant conditions. We propose C-Sul probes as optimal tools of cysteine profiling for further broadly basic research. |
| URI | http://hdl.handle.net/20.500.11897/647056 |
| ISSN | 0003-2700 |
| DOI | 10.1021/acs.analchem.1c05129 |
| Indexed | SCI(E) |
| Appears in Collections: | 化学生物学与生物技术学院 |
