| Title | SPECT Imaging of Acute Disc Herniation by Targeting Integrin alpha 5 beta 1 in Rat Models |
| Authors | Guan, Jian Yuan, Chenghua Tian, Xin Cheng, Lei Gao, Hannan Yao, Qingyu Wang, Xinyu Wu, Hao Chen, Zan Jian, Fengzeng |
| Affiliation | Capital Med Univ, Xuanwu Hosp, Dept Neurosurg, Beijing, Peoples R China China Int Neurosci Inst CHINA INI, Spine Ctr, Beijing, Peoples R China Capital Med Univ, Beijing Inst Brain Disorders, Res Ctr Spine & Spinal Cord, Beijing, Peoples R China Xuanwu Hosp, Natl Ctr Neurol Disorders, Beijing, Peoples R China Capital Med Univ, Xuanwu Hosp, Ctr Expt Anim, Beijing, Peoples R China Peking Univ, Med Isotopes Res Ctr, Sch Basic Med Sci, Beijing, Peoples R China Peking Univ, Sch Basic Med Sci, Dept Radiat Med, State Key Lab Nat & Biomimet Drugs, Beijing, Peoples R China |
| Keywords | LOW-BACK-PAIN EXTRACELLULAR-MATRIX NUCLEUS PULPOSUS FIBRONECTIN CELLS MECHANOTRANSDUCTION DEGENERATION APOPTOSIS PATHWAYS BURDEN |
| Issue Date | 9-May-2022 |
| Publisher | FRONTIERS IN NEUROLOGY |
| Abstract | Objective: Traditional morphological imaging of intervertebral disc herniation (IVDH) is challenging in early disease diagnosis. Aiming at the early diagnosis of IVD by non-invasive molecular imaging targeting of integrin alpha 5 beta 1, we performed novel imaging in rats with acute IVDH for the first time.& nbsp;Methods: Animal models were prepared by conducting an established needle puncture procedure through the normal intervertebral disc (IVD). The disc-injured rats underwent SPECT/CT imaging of the Tc-99m-3PisoDGR2 peptide at 1 day to 2 months postinjury. The expression change of integrin alpha 5 beta 1 was determined by anti-integrin alpha 5 and anti-integrin alpha 5 beta 1 immunohistochemistry (IHC). Magnetic resonance imaging (MRI) was performed for comparison during disease progression. The morphological changes of the disc were determined by safranin-O staining.& nbsp;Results: Rats with acute IVDH showed gradually increased disc uptake of Tc-99m-3PisoDGR2 from 1 to 7 days posttreatment, which was a significantly higher level than that of the normal disks in degenerative diseases. IHC results showed the expression of integrin alpha 5 beta 1 on the surface of annulus fibrosus (AF) cells and nucleus pulposus (NP) cells, which agreed with the uptake data. MRI showed a progressively decreased T2 density and MRI index throughout the investigation. Hematoxylin and eosin (HE) staining and safranin-O staining revealed a disorganized structure of the IVD as well as loss of proteoglycans after puncture.& nbsp;Conclusions: The present study demonstrated a good correlation between integrin alpha 5 beta 1 expression and acute disc herniation. The SPECT/CT imaging of Tc-99m-3PisoDGR2 targeting integrin alpha 5 beta 1 may diagnose IVDH in an acute phase for early disease management. |
| URI | http://hdl.handle.net/20.500.11897/646788 |
| ISSN | 1664-2295 |
| DOI | 10.3389/fneur.2022.782967 |
| Indexed | SCI(E) |
| Appears in Collections: | 基础医学院 天然药物与仿生药物国家重点实验室 |
