| Title | Morphological and genomic characteristics of two novel halotolerant actinomycetes, Tomitella gaofuii sp. nov. and Tomitella fengzijianii sp. nov. isolated from bat faeces |
| Authors | Huang, Yuyuan Yang, Jing Lu, Shan Lai, Xin-He Jin, Dong Zhou, Juan Zhang, Sihui Huang, Qianni Lv, Xianglian Zhu, Wentao Pu, Ji Huang, Ying Liu, Liyun Xu, Jianguo |
| Affiliation | Natl Inst Communicable Dis Control & Prevent, Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China Fudan Univ, Shanghai Inst Emerging & Reemerging Infect Dis, Shanghai Publ Hlth Clin Ctr, Shanghai 201508, Peoples R China Chinese Acad Med Sci, Res Units Discovery Unknown Bacteria & Funct, Beijing 102206, Peoples R China Shangqiu Normal Univ, Coll Chem & Chem Engn, Henan Key Lab Biomol Recognit & Sensing, Henan Joint Int Res Lab Chemo Biosensing & Early D, Shangqiu 476000, Peoples R China Peking Univ, Sch Publ Hlth, Dept Epidemiol & Biostat, Beijing 100191, Peoples R China Guangxi Med Univ, Guangxi Key Lab AIDS Prevent & Treatment, Nanning 530021, Guangxi, Peoples R China Shanxi Med Univ, Sch Publ Hlth, Dept Epidemiol, Taiyuan 030001, Peoples R China Nankai Univ, Res Inst Publ Hlth, Tianjin 300350, Peoples R China Guangxi Med Univ, Guangxi Collaborat Innovat Ctr Biomed, Sch Publ Hlth, Nanning 530021, Guangxi, Peoples R China |
| Keywords | DNA-DNA HYBRIDIZATION GEN. NOV. SPECIES DEFINITION TREES RESUSCITATION EVOLUTION SEQUENCES FAMILY GROWTH MEMBER |
| Issue Date | Apr-2022 |
| Publisher | SYSTEMATIC AND APPLIED MICROBIOLOGY |
| Abstract | Four white-pigmented, Gram-staining-positive, strictly aerobic, non-spore-forming, irregular rod-shaped bacteria were isolated from the faeces of bats collected from Guangxi autonomous region (22?degrees 20'54 '' N, 106?degrees 49'20 '' E; July 28, 2011) and Chongqing city (30?degrees 02'15 '' N, 107?degrees 07'4 '' E; September 1, 2011) of South China. The strains shared 99.3-99.9% 16S rRNA gene sequence similarity by BLAST search among them, and belonged to genus Tomitella according to 16S rRNA gene and genomic sequence-based phylogenetic/-phylogenomic analyses. Strains HY172(T) and HY188(T) contained meso-diaminopimelic acid as the diagnos-tic diamino acid, and arabinose, glucose, galactose or ribose in their whole-cell hydrolysates. Besides sharing phosphatidylethanolamine, diphosphatidylglycerol and unidentified glycolipid(s) in their polar lipid profiles, additionally HY172(T) had one unidentified phosphoglycolipid and three unidentified phos-pholipids whereas HY188(T) had phosphatidyl inositol mannoside and four unidentified aminolipids. The main cellular fatty acids of strains HY172(T) and HY188(T)& nbsp;were C-16:0, C-18:0 10-methyl, C-18:1 omega 9c and summed feature 3. The genomic DNA G + C contents of both strains (HY172(T) and HY188(T)) were 70.9 %. The genus Tomitella contains 2311 core genes, and resuscitation promoting factor (rpf) genes can be found in all members of Tomitella. The digital DNA-DNA hybridization and average nucleotide identity values of the four novel strains with other members of the genus Tomitella were within the ranges of 20.1-45.2% and 74.8-91.9%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point. Based on phylogenetic, chemotaxonomic and phenotypic analyses, these four strains could be clas-sified as two novel species of the genus Tomitella, for which the names Tomitella gaofuii sp. nov. and Tomitella fengzijianii sp. nov. are proposed. The type strains are HY172(T)& nbsp;(= CGMCC 1.18701(T)& nbsp;= JCM 34231(T)) and HY188(T)& nbsp;(= CGMCC 1.16971(T)& nbsp;= JCM 33467(T)), respectively. (C)& nbsp;2022 Elsevier GmbH. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/642807 |
| ISSN | 0723-2020 |
| DOI | 10.1016/j.syapm.2022.126294 |
| Indexed | SCI(E) |
| Appears in Collections: | 公共卫生学院 |
