| Title | Tuning the surface potential to reprogram immune microenvironment for bone regeneration |
| Authors | Li, Mei Chu, Xiao Wang, Donghui Jian, Linjia Liu, Lidan Yao, Mengyu Zhang, Dongdong Zheng, Yufeng Liu, Xuanyong Zhang, Yu Peng, Feng |
| Affiliation | Guangdong Prov Peoples Hosp, Guangdong Acad Med Sci, Med Res Ctr, Dept Orthoped, Guangzhou 510080, Peoples R China Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China Hebei Univ Technol, Sch Hlth Sci & Biomed Engn, Tianjin 300130, Peoples R China Peking Univ, Sch Mat Sci & Engn, Beijing 100871, Peoples R China |
| Keywords | ANGIOGENESIS OSTEOGENESIS IMPLANTS SUBTYPE |
| Issue Date | Mar-2022 |
| Publisher | BIOMATERIALS |
| Abstract | The induction of a suitable immune microenvironment by implant is essential for fast bone regeneration. Surface potential is a critical factor that influences immune cells behavior. We apply polydopamine coatings on a titanium (Ti) surface to decrease its surface potential. A lower surface potential favored the expression of adhesionrelated genes in bone marrow-derived monocytes (BMDMs) by activating the focal adhesion kinase signaling pathway. A lower negative surface potential results in higher electronic repulsion between the surface and the BMDMs, because the cells exhibit negative charged membrane. To resist the repulsive force, Integrin beta 1 and Integrin beta 3 in the cell membrane for low surface potential group are upregulated. Furthermore, BMDMs cultured on Ti with low surface potential are more inclined polarize towards anti-inflammatory phenotype (M2) in vitro and in vivo. Whole gene expression analysis reveals that inhibition of the PI3K-Akt-mTOR signaling axis is responsible for the immune regulation ability of Ti with low surface potential. The cytokines secreted by M2 BMDMs promote osteogenic differentiation of a mouse embryo cell line (C3H10T1/2) and increase osteointegration between the implant and newly formed bone. These findings reveal that surface potential regulation is a promising strategy to reprogram the immune microenvironment for bone regeneration and provide insights into developing biomaterials with immunomodulatory functions. |
| URI | http://hdl.handle.net/20.500.11897/642141 |
| ISSN | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2022.121408 |
| Indexed | SCI(E) |
| Appears in Collections: | 数学科学学院 |
