| Title | Analysis of TCR Repertoire by High-Throughput Sequencing Indicates the Feature of T Cell Immune Response after SARS-CoV-2 Infection |
| Authors | Wang, Yifan Duan, Fugang Zhu, Zhu Yu, Meng Jia, Xiaodong Dai, Hui Wang, Pingzhang Qiu, Xiaoyan Lu, Yinying Huang, Jing |
| Affiliation | Peking Univ, Sch Basic Med Sci, Dept Immunol, 38 Xueyuan Rd, Beijing 100191, Peoples R China Peking Univ, NHC Key Lab Med Immunol, Beijing 100191, Peoples R China Chinese Acad Med Sci, Key Lab Mol Immunol, Beijing 100191, Peoples R China Peoples Liberat Army Gen Hosp, Med Ctr 5, Comprehens Liver Canc Ctr, Beijing 100039, Peoples R China |
| Keywords | GENERATION |
| Issue Date | Jan-2022 |
| Publisher | CELLS |
| Abstract | Coronavirus disease 2019 (COVID-19) is a global infectious disease caused by the SARS-CoV-2 coronavirus. T cells play an essential role in the body's fighting against the virus invasion, and the T cell receptor (TCR) is crucial in T cell-mediated virus recognition and clearance. However, little has been known about the features of T cell response in convalescent COVID-19 patients. In this study, using 5 ' RACE technology and PacBio sequencing, we analyzed the TCR repertoire of COVID-19 patients after recovery for 2 weeks and 6 months compared with the healthy donors. The TCR clustering and CDR3 annotation were exploited to discover groups of patient-specific TCR clonotypes with potential SARS-CoV-2 antigen specificities. We first identified CD4(+) and CD8(+) T cell clones with certain clonal expansion after infection, and then observed the preferential recombination usage of V(D) J gene segments in CD4(+) and CD8(+) T cells of COVID-19 patients with different convalescent stages. More important, the TRBV6-5-TRBD2-TRBJ2-7 combination with high frequency was shared between CD4(+) T and CD8(+) T cells of different COVID-19 patients. Finally, we found the dominant characteristic motifs of the CDR3 sequence between recovered COVID-19 and healthy control. Our study provides novel insights on TCR in COVID-19 with different convalescent phases, contributing to our understanding of the immune response induced by SARS-CoV-2. |
| URI | http://hdl.handle.net/20.500.11897/638343 |
| DOI | 10.3390/cells11010068 |
| Indexed | SCI(E) |
| Appears in Collections: | 基础医学院 |
