| Title | In-situ active Bisphenol A-degrading microorganisms in mangrove sediments |
| Authors | Chen, Jianfei Tong, Tianli Yang, Yuyin Ke, Yanchu Chen, Xiuli Xie, Shuguang |
| Affiliation | Peking Univ, Coll Environm Sci & Engn, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100871, Peoples R China South China Inst Environm Sci SCIES, Minist Ecol & Environm MEE, Guangzhou 510655, Peoples R China |
| Keywords | MICROBIAL COMMUNITIES FUNCTIONAL GENE BIODEGRADATION DEGRADATION TETRABROMOBISPHENOL CARBON SOILS TRANSFORMATION NONYLPHENOL RHIZOSPHERE |
| Issue Date | 15-Apr-2022 |
| Publisher | ENVIRONMENTAL RESEARCH |
| Abstract | Bisphenol A (BPA), as both an endocrine disrupting compound and an important industrial material, is broadly distributed in coastal regions and may cause adverse effects on mangrove ecosystems. Although many BPA degraders have been isolated from various environments, the in-situ active BPA-degrading microorganisms in mangrove ecosystem are still unknown. In this study, DNA-based stable isotope probing in combination with high-throughput sequencing was adopted to pinpoint the microbes actually involved in BPA metabolism in mangrove sediments. Five bacterial genera were speculated to be associated with BPA degradation based on linear discriminant analysis (LDA) effect size (LEfSe) analysis, including Truepera, Methylobacterium, Novosphingobium, Rhodococcus and Rhodobacter. The in-situ BPA degraders were different between mudflat and forest sediments. The Shannon index of microbes in heavy fractions was significantly lower than that in light fractions. Besides, phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) demonstrated that the functional genes relevant to cytochrome P450, benzoate degradation, bisphenol degradation and citrate cycle were up-regulated significantly in in-situ BPA-degrading microbes. These findings greatly expanded the knowledge of indigenous BPA metabolic microorganisms in mangrove ecosystems. |
| URI | http://hdl.handle.net/20.500.11897/637726 |
| ISSN | 0013-9351 |
| DOI | 10.1016/j.envres.2021.112251 |
| Indexed | SCI(E) |
| Appears in Collections: | 环境科学与工程学院 环境模拟与污染控制国家重点联合实验室 |
