| Title | A simple and rapid method for extraction and measurement of circulating sphingolipids using LC-MS/MS: a targeted lipidomic analysis |
| Authors | Xu, Yifan Li, Haonan Han, Yiqun Wang, Teng Wang, Yanwen Gong, Jicheng Gao, Ke Chen, Wu Li, Weiju Zhang, Hongyin Wang, Junxia Qiu, Xinghua Zhu, Tong |
| Affiliation | Peking Univ, Coll Environm Sci & Engn, BIC ESAT, Beijing 100871, Peoples R China Peking Univ, Coll Environm Sci & Engn, SKL ESPC, Beijing 100871, Peoples R China Imperial Coll London, MRC Ctr Environm & Hlth, Environm Res Grp, London W12 7TA, England Chinese Ctr Dis Control & Prevent, Natl Inst Environm Hlth, Beijing 102206, Peoples R China Beijing Univ Technol, Dept Environm Sci, Key Lab Beijing Reg Air Pollut Control, Beijing 100124, Peoples R China Peking Univ, Peking Univ Hosp, Beijing 100871, Peoples R China |
| Keywords | TANDEM MASS-SPECTROMETRY PLASMA CERAMIDES QUANTIFICATION VALIDATION HEALTHY DISEASE |
| Issue Date | Mar-2022 |
| Publisher | ANALYTICAL AND BIOANALYTICAL CHEMISTRY |
| Abstract | Sphingolipids are a class of lipids with high structural diversity and biological pleiotropy. Mounting evidence supports a role for sphingolipids in regulating pathophysiology of cardiometabolic diseases, and they have been proposed as potential cardiometabolic biomarkers. Current methods for quantifying sphingolipids require laborious pretreatment and relatively large sample volumes, and cover limited species, hindering their application in epidemiological studies. Herein, we applied a time-, labor-, and sample-saving protocol simply using methanol for plasma sphingolipid extraction. It was compared with classical liquid-liquid extraction methods and showed significant advantages in terms of simplicity, sphingolipid coverage, and sample volume. By coupling the protocol with liquid chromatography using a wide-span mobile phase polarity parameter and tandem mass spectrometry operated in dynamic multiple reaction monitoring mode, 37 sphingolipids from 8 classes (sphingoid base, sphingoid base phosphate, ceramide-1-phosphate, lactosylceramide, hexosylceramide, sphingomyelin, ceramide, and dihydroceramide) were quantified within 16 min, using only 10 mu L of human plasma. The current method showed good performance in terms of linearity (R-2 > 0.99), intra- and interbatch accuracy (70-123%) and precision (RSD < 12%), matrix effect (91-121%), recovery (96-101%), analyte chemical stability (deviation < 19%), and carryover (< 16%). We successfully applied this method to quantify 33 detectable sphingolipids from 579 plasma samples of an epidemiological study within 10 days. The quantified sphingolipid concentrations were comparable with previous studies. Positive associations of ceramide C22:0/C24:0 and their precursors with homeostasis model assessment of insulin resistance suggested that the synthesis of the ceramides might be involved in insulin resistance. This novel method constitutes a simple and rapid approach to quantify circulating sphingolipids for epidemiological studies using targeted lipidomic analysis, which will help elucidate the sphingolipid-regulated pathways underlying cardiometabolic diseases. |
| URI | http://hdl.handle.net/20.500.11897/637133 |
| ISSN | 1618-2642 |
| DOI | 10.1007/s00216-021-03853-z |
| Indexed | EI SCI(E) |
| Appears in Collections: | 环境科学与工程学院 校医院 |
