| Title | Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression |
| Authors | Yang, Junjun Wang, Xin Fan, Yahan Song, Xiongbo Wu, Jiangyi Fu, Zhenlan Li, Tao Huang, Yang Tang, ZheXiong Meng, Shuo Liu, Na Chen, Jiajia Liu, Pingju Yang, Liu Gong, Xiaoyuan Chen, Cheng |
| Affiliation | Third Mil Med Univ, Southwest Hosp, Ctr Joint Surg, Army Med Univ, Chongqing 400038, Peoples R China Chongqing Med Univ, Coll Med Informat, Chongqing 400016, Peoples R China Third Mil Med Univ, Southwest Hosp, Blood Transfus Dept, Army Med Univ, Chongqing 400038, Peoples R China Peking Univ, Shenzhen Hosp, Dept Sports Med, Shenzhen 518036, Peoples R China Southwest Univ, Sch Life Sci, Key Lab Freshwater Fish Reprod & Dev, Minist Educ,Lab Mol Dev Biol, Chongqing 400038, Peoples R China Third Mil Med Univ, Biomed Anal Ctr, Army Med Univ, Chongqing 400038, Peoples R China Zunyi Tradit Chinese Med Hosp, Dept Orthoped, Zunyi 563099, Guizhou, Peoples R China |
| Keywords | MESENCHYMAL STEM-CELLS STROMAL CELLS SYNOVIAL-FLUID CARTILAGE REPAIR ATTACHMENT THERAPY DIFFERENTIATION EXPRESSION HYDROGELS DELIVERY |
| Issue Date | Apr-2022 |
| Publisher | BIOACTIVE MATERIALS |
| Abstract | Intra-articular injection of mesenchymal stem cells (MSCs) is a promising strategy for osteoarthritis (OA) treatment. However, more and more studies reveal that the injected MSCs have poor adhesion, migration, and survival in the joint cavity. A recent study shows that tropoelastin (TE) regulates adhesion, proliferation and phenotypic maintenance of MSCs as a soluble additive, indicating that TE could promote MSCs-homing in regenerative medicine. In this study, we used TE as injection medium, and compared it with classic media in MSCs intra-articular injection such as normal saline (NS), hyaluronic acid (HA), and platelet-rich plasma (PRP). We found that TE could effectively improve adhesion, migration, chondrogenic differentiation of infrapatellar fat pad MSCs (IPFP-MSCs) and enhance matrix synthesis of osteoarthritic chondrocytes (OACs) in indirect-coculture system. Moreover, TE could significantly enhance IPFP-MSCs adhesion via activation of integrin beta 1, ERK1/2 and vinculin (VCL) in vitro. In addition, intra-articular injection of TE-IPFP MSCs suspension resulted in a short-term increase in survival rate of IPFP-MSCs and better histology scores of rat joint tissues. Inhibition of integrin beta 1 or ERK1/2 attenuated the protective effect of TE-IPFP MSCs suspension in vivo. In conclusion, TE promotes performance of IPFP-MSCs and protects knee cartilage from damage in OA through enhancement of cell adhesion and activation of integrin beta 1/ERK/VCL pathway. Our findings may provide new insights in MSCs intra-articular injection for OA treatment. |
| URI | http://hdl.handle.net/20.500.11897/636887 |
| DOI | 10.1016/j.bioactmat.2021.09.011 |
| Indexed | SCI(E) |
| Appears in Collections: | 深圳医院 |
