| Title | Advances in Metagenomics and Its Application in Environmental Microorganisms |
| Authors | Zhang, Lu Chen, FengXin Zeng, Zhan Xu, Mengjiao Sun, Fangfang Yang, Liu Bi, Xiaoyue Lin, Yanjie Gao, YuanJiao Hao, HongXiao Yi, Wei Li, Minghui Xie, Yao |
| Affiliation | Capital Med Univ, Beijing Ditan Hosp, Dept Hepatol Div 2, Beijing, Peoples R China Peking Univ, Dept Hepatol Div 2, Ditan Teaching Hosp, Beijing, Peoples R China Capital Med Univ, Beijing Ditan Hosp, Dept Gynecol & Obstet, Beijing, Peoples R China |
| Keywords | ELECTROCHEMICAL SYSTEMS QUALITY-CONTROL GENOMIC DNA EXPRESSION CHALLENGES LIBRARIES CLONING IDENTIFICATION HYBRIDIZATION DIVERSITY |
| Issue Date | 17-Dec-2021 |
| Publisher | FRONTIERS IN MICROBIOLOGY |
| Abstract | Metagenomics is a new approach to study microorganisms obtained from a specific environment by functional gene screening or sequencing analysis. Metagenomics studies focus on microbial diversity, community constitute, genetic and evolutionary relationships, functional activities, and interactions and relationships with the environment. Sequencing technologies have evolved from shotgun sequencing to high-throughput, next-generation sequencing (NGS), and third-generation sequencing (TGS). NGS and TGS have shown the advantage of rapid detection of pathogenic microorganisms. With the help of new algorithms, we can better perform the taxonomic profiling and gene prediction of microbial species. Functional metagenomics is helpful to screen new bioactive substances and new functional genes from microorganisms and microbial metabolites. In this article, basic steps, classification, and applications of metagenomics are reviewed. |
| URI | http://hdl.handle.net/20.500.11897/634562 |
| DOI | 10.3389/fmicb.2021.766364 |
| Indexed | SCI(E) |
| Appears in Collections: | 北京地坛医院 |
