| Title | Fiber-optic large-depth 3D chromatic confocal endomicroscopy |
| Authors | Yang, Xuefang Wang, Ye Zhang, Hejun Qin, Haoye Wang, Siyu Tong, Yihang Zhou, Keji Sun, Rui Yue, Shuhua Chen, Xun Ding, Shigang Wang, Pu |
| Affiliation | Beihang Univ, Beijing Adv Innovat Ctr Biomed Engn, Minist Educ,Inst Med Photon, Key Lab Biomech & Mechanobiol,Sch Biol Sci & Med, Beijing 100083, Peoples R China Peking Univ, Hosp 3, Dept Gastroenterol, Beijing 100191, Peoples R China Chinese Univ Hong Kong, Shenzhen Res Inst, BJR Biomed Tech Co, Shenzhen 518057, Guangdong, Peoples R China Huazhong Univ Sci & Technol, MoE Key Lab Biomed Photon, Wuhan Natl Lab Optoelect, Wuhan 430074, Peoples R China Fujian Normal Univ, Key Lab OptoElect Sci & Technol Med, Fujian Prov Key Lab Photon Technol, Minist Educ, Fuzhou 350007, Peoples R China |
| Keywords | MICROSCOPY PROBE ENDOSCOPY DIAGNOSIS |
| Issue Date | 1-Jan-2022 |
| Publisher | BIOMEDICAL OPTICS EXPRESS |
| Abstract | Current endoscopy techniques have difficulties to provide both high resolution and large imaging depth, which significantly hinders the early diagnosis of gastric cancer. Here, we developed a label-free, large-depth, three-dimensional (3D) chromatic reflectance confocal endomicroscopy. In order to solve the problem of insufficient imaging depth of traditional chromatic confocal microscopy, a customized miniature objective lens both with large chromatic focal shift and correction for spherical aberration was used to focus light of different wavelengths at different depths of the sample simultaneously, anda fiber bundle containing 50000 single-mode cores was used to collect the confocal reflectance signal. To acquire detailed information along the axial direction at a faster speed, a high-speed multi-pixel spectrometer was used to realize simultaneous detection of multi-depth signals. Specifically, we have built up a label-free fiber-optic 3D chromatic reflectance confocal endomicroscopy, with 2.3 mu m lateral resolution, imaging depth of 570 mu m in 3D phantom and 220 mu m in tissue, and 1.5 Hz 3D volumetric frame rate. We have demonstrated that the fiber-optic 3D chromatic confocal endomicroscopy can be used to image human gastric tissues ex vivo, and provide important morphological information for diagnosis without labeling. These results show the great potential of the fiber-optic 3D chromatic confocal endomicroscopy for gastric cancer diagnosis. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
| URI | http://hdl.handle.net/20.500.11897/634420 |
| ISSN | 2156-7085 |
| DOI | 10.1364/BOE.442029 |
| Indexed | SCI(E) |
| Appears in Collections: | 第三医院 |
