| Title | Fabrication of a bio-instructive scaffold conferred with a favorable microenvironment allowing for superior implant osseointegration and accelerated in situ vascularized bone regeneration via type H vessel formation |
| Authors | He, Yijun Wang, Wenhao Lin, Shaozhang Yang, Yixi Song, Lizhi Jing, Yihan Chen, Lihao He, Zaopeng Li, Wei Xiong, Ao Yeung, Kelvin W. K. Zhao, Qi Jiang, Yuan Li, Zijie Pei, Guoxian Zhang, Zhi-Yong |
| Affiliation | Guangzhou Med Univ, Translat Res Ctr Regenerat Med & Printing 3D, Guangdong Prov Engn Res Ctr Biomed Engn, State Key Lab Resp Dis,Affiliated Hosp 3,South Ch, Guangzhou 510150, Peoples R China Guangzhou Med Univ, Hand & Foot Surg Plast Surg, Affiliated Shunde Hosp, Foshan 528315, Peoples R China Peking Univ, Dept Bone & Joint Surg, Shenzhen Hosp, Shenzhen 518036, Peoples R China Peking Univ, Natl & Local Joint Engn Res Ctr Orthopaed Biomat, Shenzhen Hosp, Shenzhen 518036, Peoples R China Univ Hong Kong, Dept Orthopaed & Traumatol, Hong Kong 999077, Peoples R China Univ Hong Kong, Shenzhen Key Lab Innovat Technol Orthopaed Trauma, Shenzhen Hosp, Shenzhen 518053, Peoples R China Southern Univ Sci & Technol, Affiliated Hosp 3, Shenzhen 518055, Peoples R China Guangzhou Med Univ, Dept Orthopaed Surg, Affiliated Hosp 3, Guangzhou 510150, Peoples R China Guangzhou Med Univ, City Key Lab, Med Technol & Related Equipment Res Spinal Injury, Affiliated Hosp 3, Guangzhou 510150, PR, Peoples R China |
| Keywords | DECELLULARIZED EXTRACELLULAR-MATRIX 3D PRINTED SCAFFOLDS STEM-CELLS OSTEOGENESIS ANGIOGENESIS DIFFERENTIATION CARTILAGE COLLAGEN REPAIR OSTEOINTEGRATION |
| Issue Date | Mar-2022 |
| Publisher | BIOACTIVE MATERIALS |
| Abstract | The potential translation of bio-inert polymer scaffolds as bone substitutes is limited by the lack of neovascularization upon implantation and subsequently diminished ingrowth of host bone, most likely resulted from the inability to replicate appropriate endogenous crosstalk between cells. Human umbilical vein endothelial cell-derived decellularized extracellular matrix (HdECM), which contains a collection of angiocrine biomolecules, has recently been demonstrated to mediate endothelial cells(ECs) - osteoprogenitors(OPs) crosstalk. We employed the HdECM to create a PCL (polycaprolactone)/fibrin/HdECM (PFE) hybrid scaffold. We hypothesized PFE scaffold could reconstitute a bio-instructive microenvironment that reintroduces the crosstalk, resulting in vascularized bone regeneration. Following implantation in a rat femoral bone defect, the PFE scaffold demonstrated early vascular infiltration and enhanced bone regeneration by microangiography (mu-AG) and microcomputational tomography (mu-CT). Based on the immunofluorescence studies, PFE mediated the endogenous angiogenesis and osteogenesis with a substantial number of type H vessels and osteoprogenitors. In addition, superior osseointegration was observed by a direct host bone-PCL interface, which was likely attributed to the formation of type H vessels. The bio-instructive microenvironment created by our innovative PFE scaffold made possible superior osseointegration and type H vessel-related bone regeneration. It could become an alternative solution of improving the osseointegration of bone substitutes with the help of induced type H vessels, which could compensate for the inherent biological inertness of synthetic polymers. |
| URI | http://hdl.handle.net/20.500.11897/634367 |
| DOI | 10.1016/j.bioactmat.2021.07.030 |
| Indexed | SCI(E) |
| Appears in Collections: | 深圳医院 |
