| Title | Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen |
| Authors | Toh, Shigeo Takata, Naoki Ando, Eigo Toda, Yosuke Wang, Yin Hayashi, Yuki Mitsuda, Nobutaka Nagano, Soichiro Taniguchi, Toru Kinoshita, Toshinori |
| Affiliation | Meijo Univ, Dept Environm Biosci, Nagoya, Aichi, Japan Nagoya Univ, Grad Sch Sci, Div Biol Sci, Nagoya, Aichi, Japan Forestry & Forest Prod Res Inst, Forest Biores Ctr, Hitachi, Ibaraki, Japan Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo, Japan Japan Sci & Technol Agcy, Saitama, Japan Nagoya Univ, Inst Transformat Biomol WPI ITbM, Nagoya, Aichi, Japan Phytometrics Co Ltd, Shizuoka, Japan Nagoya Univ, Inst Adv Res, Nagoya, Aichi, Japan Peking Univ, Inst Ecol, Coll Urban & Environm Sci, Beijing, Peoples R China Peking Univ, Key Lab Earth Surface Proc, Minist Educ, Beijing, Peoples R China Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki, Japan Natl Inst Adv Ind Sci & Technol, Global Zero Emiss Res Ctr, Tsukuba, Ibaraki, Japan Forestry & Forest Prod Res Inst, Forest Tree Breeding Ctr, Hitachi, Ibaraki, Japan Forestry & Forest Prod Res Inst, Forest Tree Breeding Ctr, Tohoku Reg Breeding Off, Takizawa, Japan |
| Keywords | INDUCED PHOSPHORYLATION BIOMASS PRODUCTION BLUE EXPRESSION CARBON GENES TREES RED |
| Issue Date | 26-Nov-2021 |
| Publisher | FRONTIERS IN PLANT SCIENCE |
| Abstract | Stomata in the plant epidermis open in response to light and regulate CO2 uptake for photosynthesis and transpiration for uptake of water and nutrients from roots. Light-induced stomatal opening is mediated by activation of the plasma membrane (PM) H+-ATPase in guard cells. Overexpression of PM H+-ATPase in guard cells promotes light-induced stomatal opening, enhancing photosynthesis and growth in Arabidopsis thaliana. In this study, transgenic hybrid aspens overexpressing Arabidopsis PM H+-ATPase (AHA2) in guard cells under the strong guard cell promoter Arabidopsis GC1 (AtGC1) showed enhanced light-induced stomatal opening, photosynthesis, and growth. First, we confirmed that AtGC1 induces GUS expression specifically in guard cells in hybrid aspens. Thus, we produced AtGC1::AHA2 transgenic hybrid aspens and confirmed expression of AHA2 in AtGC1::AHA2 transgenic plants. In addition, AtGC1::AHA2 transgenic plants showed a higher PM H+-ATPase protein level in guard cells. Analysis using a gas exchange system revealed that transpiration and the photosynthetic rate were significantly increased in AtGC1::AHA2 transgenic aspen plants. AtGC1::AHA2 transgenic plants showed a>20% higher stem elongation rate than the wild type (WT). Therefore, overexpression of PM H+-ATPase in guard cells promotes the growth of perennial woody plants. |
| URI | http://hdl.handle.net/20.500.11897/632818 |
| ISSN | 1664-462X |
| DOI | 10.3389/fpls.2021.766037 |
| Indexed | SCI(E) |
| Appears in Collections: | 城市与环境学院 地表过程分析与模拟教育部重点实验室 |
