| Title | Carbon Nanotube Field-Effect Transistor Biosensor for Ultrasensitive and Label-Free Detection of Breast Cancer Exosomal miRNA21 |
| Authors | Li, Tingxian Liang, Yuqi Li, Jiahao Yu, Yi Xiao, Meng-Meng Ni, Wei Zhang, Zhiyong Zhang, Guo-Jun |
| Affiliation | Hubei Univ Chinese Med, Sch Lab Med, Wuhan 430065, Peoples R China Peking Univ, Dept Elect, Key Lab Phys & Chem Nanodevices, Beijing 100871, Peoples R China Peking Univ, Dept Elect, Ctr Carbon Based Elect, Beijing 100871, Peoples R China Hubei Prov Hosp Tradit Chinese Med, Wuhan 430061, Peoples R China Xiangtan Univ, Hunan Inst Adv Sensing & Informat Technol, Xiangtan 411105, Hunan, Peoples R China |
| Keywords | CIRCULATING MICRORNAS ELECTRICAL DETECTION DNA |
| Issue Date | 23-Nov-2021 |
| Publisher | ANALYTICAL CHEMISTRY |
| Abstract | Tumor-derived exosomal miRNAs may have important functions in the onset and progression of cancers and are potential biomarkers for early diagnosis and prognosis monitoring. Yet, simple, sensitive, and label-free detection of exosomal miRNAs remains challenging. Herein, an ultrasensitive, label-free, and stable field-effect transistor (FET) biosensor based on a polymer-sorted high-purity semiconducting carbon nanotube (CNT) film is reported to detect exosomal miRNA. Different from conventional CNT FETs, the CNT FET biosensors employed a floating gate structure using an ultrathin Y2O3 as an insulating layer, and assembled Au nanoparticles (AuNPs) on Y2O3 as linkers to anchor probe molecules. A thiolated oligonucleotide probe was immobilized on the AuNP surface of the sensing area, after which miRNA21 was detectable by monitoring the current change before and after hybridization between the immobilized DNA probe and target miRNA. This method achieved both high sensitivity (LOD: 0.87 aM) and high specificity. Furthermore, the FET biosensor was employed to test clinical plasma samples, showing significant differences between healthy people and breast cancer patients. The CNT FET biosensor shows the potential applications in the clinical diagnosis of breast cancer. |
| URI | http://hdl.handle.net/20.500.11897/632741 |
| ISSN | 0003-2700 |
| DOI | 10.1021/acs.analchem.1c03573 |
| Indexed | SCI(E) |
| Appears in Collections: | 信息科学技术学院 纳米器件物理与化学教育部重点实验室 |
