| Title | Development and evaluation of a simultaneous strategy for pyrimidine metabolome quantification in multiple biological samples |
| Authors | Xu, Feng Pang, Yuanyuan Nie, Qixing Zhang, Zhipeng Ye, Chuan Jiang, Changtao Wang, Yuan Liu, Huiying |
| Affiliation | Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing, Peoples R China Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China Peking Univ, Hosp 3, Ctr Basic Med Res, Inst Med Innovat & Res, Beijing, Peoples R China Peking Univ, Ctr Obes & Metab Dis Res, Sch Basic Med Sci, Beijing, Peoples R China Peking Univ, Hosp 3, Gen Surg Dept, Beijing, Peoples R China Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing, Peoples R China |
| Keywords | NUCLEOTIDE BIOSYNTHESIS URIDINE STIMULATION IMPAIRMENT CYTIDINE PLASMA ACID |
| Issue Date | 30-Mar-2022 |
| Publisher | FOOD CHEMISTRY |
| Abstract | Pyrimidines are critical nutrients and key biomolecules in nucleic acid biosynthesis and carbohydrate and lipid metabolism. Here, we proposed the concept of the pyrimidine metabolome, which covers 14 analytes in pyrimidine de novo and salvage synthetic pathways, and established a novel analytical strategy with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to efficiently illustrate pyrimidine transient distribution and dynamic balance. The lower limits of quantification (LLOQs) of all analytes were less than 10 ng/mL. Acceptable inter-and intra-day relative deviation (<15%) was detected, and good stability was obtained under different storage conditions. Metabolomics analysis revealed pyrimidine metabolic diversity in the plasma and brain among species, and a visualization strategy exhibited that pyrimidine biosynthetic metabolism is quite active in brain. Distinct metabolic features were also observed in cells with pyrimidine metabolomic disorders during proliferation and apoptosis. Absolute concentrations of pyrimidine metabolites in different bio-samples offered reference data for future pyrimidine studies. |
| URI | http://hdl.handle.net/20.500.11897/632110 |
| ISSN | 0308-8146 |
| DOI | 10.1016/j.foodchem.2021.131405 |
| Indexed | EI SCI(E) |
| Appears in Collections: | 基础医学院 第三医院 药学院 天然药物与仿生药物国家重点实验室 |
