| Title | Mapping and Characterization of a Wheat Stem Rust Resistance Gene in Durum Wheat "Kronos" |
| Authors | Li, Hongna Hua, Lei Rouse, Matthew N. Li, Tianya Pang, Shuyong Bai, Shengsheng Shen, Tao Luo, Jing Li, Hongyu Zhang, Wenjun Wang, Xiaodong Dubcovsky, Jorge Chen, Shisheng |
| Affiliation | Peking Univ, Inst Adv Agr Sci, Weifang, Peoples R China Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA Shenyang Agr Univ, Coll Plant Protect, Shenyang, Peoples R China Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA Howard Hughes Med Inst, Chevy Chase, MD 20815 USA Hebei Agr Univ, Coll Plant Protect, State Key Lab North China Crop Improvement & Regu, Baoding, Peoples R China |
| Keywords | F-SP TRITICI PUCCINIA-GRAMINIS CONFERS RESISTANCE FOOD SECURITY LINKAGE MAPS RACE UG99 IDENTIFICATION SR13 INHERITANCE EMERGENCE |
| Issue Date | 15-Oct-2021 |
| Publisher | FRONTIERS IN PLANT SCIENCE |
| Abstract | Wheat stem (or black) rust is one of the most devastating fungal diseases, threatening global wheat production. Identification, mapping, and deployment of effective resistance genes are critical to addressing this challenge. In this study, we mapped and characterized one stem rust resistance (Sr) gene from the tetraploid durum wheat variety Kronos (temporary designation SrKN). This gene was mapped on the long arm of chromosome 2B and confers resistance to multiple virulent Pgt races, such as TRTTF and BCCBC. Using a large mapping population (3,366 gametes), we mapped SrKN within a 0.29 cM region flanked by the sequenced-based markers pku4856F2R2 and pku4917F3R3, which corresponds to 5.6- and 7.2-Mb regions in the Svevo and Chinese Spring reference genomes, respectively. Both regions include a cluster of nucleotide binding leucine-repeat (NLR) genes that likely includes the candidate gene. An allelism test failed to detect recombination between SrKN and the previously mapped Sr9e gene. This result, together with the similar seedling resistance responses and resistance profiles, suggested that SrKN and Sr9e may represent the same gene. We introgressed SrKN into common wheat and developed completely linked markers to accelerate its deployment in the wheat breeding programs. SrKN can be a valuable component of transgenic cassettes or gene pyramids that includes multiple resistance genes to control this devastating disease. |
| URI | http://hdl.handle.net/20.500.11897/629162 |
| ISSN | 1664-462X |
| DOI | 10.3389/fpls.2021.751398 |
| Indexed | SCI(E) |
| Appears in Collections: | 待认领 |
