| Title | Identification and characterization of Sr22b, a new allele of the wheat stem rust resistance gene Sr22 effective against the Ug99 race group |
| Authors | Luo, Jing Rouse, Matthew N. Hua, Lei Li, Hongna Li, Boshu Li, Tianya Zhang, Wenjun Gao, Caixia Wang, Yanpeng Dubcovsky, Jorge Chen, Shisheng |
| Affiliation | Peking Univ, Inst Adv Agr Sci, Weifang 261000, Shandong, Peoples R China Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA Chinese Acad Sci, Innovat Acad Seed Design, Ctr Genome Editing, Inst Genet & Dev Biol,State Key Lab Plant Cell &, Beijing, Peoples R China Shenyang Agr Univ, Coll Plant Protect, Shenyang 110000, Liaoning, Peoples R China Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA Howard Hughes Med Inst, Chevy Chase, MD 20815 USA |
| Keywords | F-SP TRITICI BIOLISTIC TRANSFORMATION CHROMOSOME LOCATION CONFERS RESISTANCE SPRING WHEAT DURUM-WHEAT PROTEIN MONOCOCCUM EMERGENCE THREAT |
| Issue Date | Nov-2021 |
| Publisher | PLANT BIOTECHNOLOGY JOURNAL |
| Abstract | Wheat stem (or black) rust, caused by Puccinia graminis f. sp. tritici (Pgt), has been historically among the most devastating global fungal diseases of wheat. The recent occurrence and spread of new virulent races such as Ug99 have prompted global efforts to identify and isolate more effective stem rust resistance (Sr) genes. Here, we report the map-based cloning of the Ug99-effective SrTm5 gene from diploid wheat Triticum monococcum accession PI 306540 that encodes a typical coiled-coil nucleotide-binding leucine-rich repeat protein. This gene, designated as Sr22b, is a new allele of Sr22 with a rare insertion of a large (13.8-kb) retrotransposon into its second intron. Biolistic transformation of an similar to 112-kb circular bacterial artificial chromosome plasmid carrying Sr22b into the susceptible wheat variety Fielder was sufficient to confer resistance to stem rust. In a survey of 168 wheat genotypes, Sr22b was present only in cultivated T. monococcum subsp. monococcum accessions but absent in all tested tetraploid and hexaploid wheat lines. We developed a diagnostic molecular marker for Sr22b and successfully introgressed a T. monococcum chromosome segment containing this gene into hexaploid wheat to accelerate its deployment and pyramiding with other Sr genes in wheat breeding programmes. Sr22b can be a valuable component of gene pyramids or transgenic cassettes combining different resistance genes to control this devastating disease. |
| URI | http://hdl.handle.net/20.500.11897/629144 |
| ISSN | 1467-7644 |
| DOI | 10.1111/pbi.13737 |
| Indexed | SCI(E) |
| Appears in Collections: | 待认领 |
