| Title | Bringing together the best of chemistry and biology: hybrid indicators for imaging neuronal membrane potential |
| Authors | Liu, Shuzhang Yang, Junqi Zou, Peng |
| Affiliation | Peking Univ, Minist Educ, Coll Chem & Mol Engn,Synthet & Funct Biomol Ctr, Beijing Natl Lab Mol Sci,Key Lab Bioorgan Chem &, Beijing 100871, Peoples R China Peking Univ, Peking Tsinghua NIBS Joint Grad Program, Beijing 100871, Peoples R China Peking Univ, PKU IDG McGovern Inst Brain Res, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China Chinese Inst Brain Res, Beijing 102206, Peoples R China |
| Keywords | ELECTRICAL-ACTIVITY VOLTAGE INDICATOR IN-VIVO PROTEIN CELL FLUORESCENCE BRIGHT |
| Issue Date | 1-Nov-2021 |
| Publisher | JOURNAL OF NEUROSCIENCE METHODS |
| Abstract | Membrane potential is an indispensable biophysical signal in neurobiology. Imaging neuronal electrical signals with fluorescent indicators allows for non-invasive recording at high spatial resolution. Over the past decades, both genetically encoded voltage indicators (GEVIs) and organic voltage sensing dyes (OVSDs) have been developed to achieve imaging membrane potential dynamics in cultured neurons and in vivo. More recently, hybrid voltage indicators have gained increasing attention due to their superior fluorescent quantum yield and photostability as compared to conventional GEVIs. In this mini-review, we summarize the design, characterization and biological applications of hybrid voltage indicators, and discuss future improvements. |
| URI | http://hdl.handle.net/20.500.11897/628629 |
| ISSN | 0165-0270 |
| DOI | 10.1016/j.jneumeth.2021.109348 |
| Indexed | SCI(E) |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ 生物有机与分子工程教育部重点实验室 生命科学学院 |
