| Title | Complete Mapping of DNA-Protein Interactions at the Single-Molecule Level |
| Authors | Liu, Wenzhe Li, Jie Xu, Yongping Yin, Dongbao Zhu, Xin Fu, Huanyan Su, Xiaodong Guo, Xuefeng |
| Affiliation | Peking Univ, Coll Chem & Mol Engn, Natl Biomed Imaging Ctr, Beijing Natl Lab Mol Sci,State Key Lab Struct Che, Beijing 100871, Peoples R China Shenzhen Bay Lab, Shenzhen 518132, Peoples R China Peking Univ, Biomed Pioneering Innovat Ctr BIOPIC, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China Nankai Univ, Coll Elect Informat & Opt Engn, Inst Modern Opt, Frontiers Sci Ctr New Organ Matter,Ctr Single Mol, 38 Tongyan Rd, Tianjin 300350, Peoples R China |
| Keywords | ONE-DIMENSIONAL DIFFUSION REPRESSOR-OPERATOR INTERACTION DRIVEN MECHANISMS RNA-POLYMERASE NUCLEIC-ACIDS LABEL-FREE TRANSCRIPTION SEARCH TRANSLOCATION DYNAMICS |
| Issue Date | Oct-2021 |
| Publisher | ADVANCED SCIENCE |
| Abstract | DNA-protein interaction plays an essential role in the storage, expression, and regulation of genetic information. A 1D/3D facilitated diffusion mechanism has been proposed to explain the extraordinarily rapid rate of DNA-binding protein (DBP) searching for cognate sequence along DNA and further studied by single-molecule experiments. However, direct observation of the detailed chronological protein searching image is still a formidable challenge. Here, for the first time, a single-molecule electrical monitoring technique is utilized to realize label-free detection of the DBP-DNA interaction process based on high-gain silicon nanowire field-effect transistors (SiNW FETs). The whole binding process of WRKY domain and DNA has been visualized with high sensitivity and single-base resolution. Impressively, the swinging of hydrogen bonds between amino acid residues and bases in DNA induce the dynamic collective motion of DBP-DNA. This in situ, label-free electrical detection platform provides a practical experimental methodology for dynamic studies of various biomolecules. |
| URI | http://hdl.handle.net/20.500.11897/626415 |
| DOI | 10.1002/advs.202101383 |
| Indexed | SCI(E) |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ 生物医学前沿创新中心 |
