| Title | Protease Inhibitor-Dependent Inhibition of Light-Induced Stomatal Opening |
| Authors | Wang, Tenghua Ye, Wenxiu Wang, Yin Zhang, Maoxing Aihara, Yusuke Kinoshita, Toshinori |
| Affiliation | Nagoya Univ, Grad Sch Sci, Nagoya, Aichi, Japan Kyoto Univ, Grad Sch Sci, Kyoto, Japan Nagoya Univ, Inst Transformat Biomol WPI ITbM, Nagoya, Aichi, Japan Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai, Peoples R China Peking Univ, Coll Urban & Environm Sci, Beijing, Peoples R China Peking Univ, Key Lab Earth Surface Proc, Minist Educ, Inst Ecol, Beijing, Peoples R China Foshan Univ, Int Res Ctr Environm Membrane Biol, Dept Hort, Foshan, Peoples R China |
| Keywords | MEMBRANE H+-ATPASE SMALL-MOLECULE INHIBITORS ABSCISIC-ACID BLUE-LIGHT GUARD-CELLS ARABIDOPSIS PHOSPHORYLATION FAMILY SIGNAL HOMOLOGS |
| Issue Date | 10-Sep-2021 |
| Publisher | FRONTIERS IN PLANT SCIENCE |
| Abstract | Stomata in the epidermis of plants play essential roles in the regulation of photosynthesis and transpiration. Stomata open in response to blue light (BL) by phosphorylation-dependent activation of the plasma membrane (PM) H+-ATPase in guard cells. Under water stress, the plant hormone abscisic acid (ABA) promotes stomatal closure via the ABA-signaling pathway to reduce water loss. We established a chemical screening method to identify compounds that affect stomatal movements in Commelina benghalensis. We performed chemical screening using a protease inhibitor (PI) library of 130 inhibitors to identify inhibitors of stomatal movement. We discovered 17 PIs that inhibited light-induced stomatal opening by more than 50%. Further analysis of the top three inhibitors (PI1, PI2, and PI3; inhibitors of ubiquitin-specific protease 1, membrane type-1 matrix metalloproteinase, and matrix metalloproteinase-2, respectively) revealed that these inhibitors suppressed BL-induced phosphorylation of the PM H+-ATPase but had no effect on the activity of phototropins or ABA-dependent responses. The results suggest that these PIs suppress BL-induced stomatal opening at least in part by inhibiting PM H+-ATPase activity but not the ABA-signaling pathway. The targets of PI1, PI2, and PI3 were predicted by bioinformatics analyses, which provided insight into factors involved in BL-induced stomatal opening. |
| URI | http://hdl.handle.net/20.500.11897/626069 |
| ISSN | 1664-462X |
| DOI | 10.3389/fpls.2021.735328 |
| Indexed | SCI(E) |
| Appears in Collections: | 城市与环境学院 地表过程分析与模拟教育部重点实验室 |
