| Title | Atypical laminin spots and pull-generated microtubule-actin projections mediate Drosophila wing adhesion |
| Authors | Sun, Tianhui Song, Yuzhao Teng, Dequn Chen, Yanan Dai, Jianli Ma, Mengqi Zhang, Wei Pastor-Pareja, Jose C. |
| Affiliation | Tsinghua Univ, Sch Life Sci, Beijing, Peoples R China Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Beijing, Peoples R China Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing, Peoples R China Peking Univ, Peking Tsinghua Ctr Life Sci, Acad Adv Interdisciplinary Studies, Beijing, Peoples R China Univ Med Ctr, Inst Neuropathol, Gottingen, Germany Baylor Coll Med, Dept Mol & Human Genet, Howard Hughes Med Inst, Houston, TX 77030 USA |
| Keywords | DISC EVERSION COLLAGEN-IV F-ACTIN GENE TISSUE SHAPE METAMORPHOSIS MORPHOGENESIS INTEGRINS FILAMENTS |
| Issue Date | 7-Sep-2021 |
| Publisher | CELL REPORTS |
| Abstract | During Drosophila metamorphosis, dorsal and ventral wing surfaces adhere, separate, and reappose in a paradoxical process involving cell-matrix adhesion, matrix production and degradation, and long cellular projections. The identity of the intervening matrix, the logic behind the adhesion-reapposition cycle, and the role of projections are unknown. We find that laminin matrix spots devoid of other main basement membrane components mediate wing adhesion. Through live imaging, we show that long microtubule-actin cables grow from those adhesion spots because of hydrostatic pressure that pushes wing surfaces apart. Formation of cables resistant to pressure requires spectraplakin, Patronin, septins, and Sdb, a SAXO1/2 microtubule stabilizer expressed under control of wing intervein-selector SRF. Silkworms and dead-leaf butterflies display similar dorso-ventral projections and expression of Sdb in intervein SRF-like patterns. Our study supports the morphogenetic importance of atypical basement-membrane-related matrices and dissects matrix-cytoskeleton coordination in a process of great evolutionary significance. |
| URI | http://hdl.handle.net/20.500.11897/624458 |
| ISSN | 2211-1247 |
| DOI | 10.1016/j.celrep.2021.109667 |
| Indexed | SCI(E) |
| Appears in Collections: | 生命科学学院 |
