| Title | Defective mitochondrial function by mutation in THICK ALEURONE 1 encoding a mitochon-driontargeted single-stranded DNA-binding protein leads to increased aleurone cell layers and improved nutrition in rice |
| Authors | Li, Dong-Qi Wu, Xiao-Ba Wang, Hai-Feng Feng, Xue Yan, Shi-Juan Wu, Sheng-Yang Liu, Jin-Xin Yao, Xue-Feng Bai, Ai-Ning Zhao, Heng Song, Xiu-Fen Guo, Lin Zhang, Shi-Yong Liu, Chun-Ming |
| Affiliation | Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China Univ Chinese Acad Sci, Beijing 100864, Peoples R China Shandong Acad Agr Sci, Rice Res Inst, Jinan 250100, Peoples R China Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preser, Guangzhou 510640, Peoples R China Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China |
| Keywords | ORYZA-SATIVA-L MAIZE ENDOSPERM TRANSCRIPTION FACTORS ALTERNATIVE OXIDASE EXPRESSION ANALYSIS MOLECULAR-CLONING GENE-EXPRESSION DYNAMIC CHANGES OPAQUE-2 MAIZE CARYOPSIS |
| Issue Date | 2-Aug-2021 |
| Publisher | MOLECULAR PLANT |
| Abstract | Cereal endosperm comprises an outer aleurone and an inner starchy endosperm. Although these two tissues have the same developmental origin, they differ in morphology, cell fate, and storage product accumulation, with the mechanism largely unknown. Here, we report the identification and characterization of rice thick aleurone 1 (ta1) mutant that shows an increased number of aleurone cell layers and increased contents of nutritional factors including proteins, lipids, vitamins, dietary fibers, and micronutrients. We identified that the TA1 gene, which is expressed in embryo, aleurone, and subaleurone in caryopses, encodes a mitochondrion-targeted protein with single-stranded DNA-binding activity named OsmtSSB1. Cytological analyses revealed that the increased aleurone cell layers in ta1 originate from a developmental switch of subaleurone toward aleurone instead of starchy endosperm in the wild type. We found that TA1/OsmtSSB1 interacts with mitochondrial DNA recombinase RECA3 and DNA helicase TWINKLE, and down-regulation of RECA3 or TWINKLE also leads to ta1-like phenotypes. We further showed that mutation in TA1/OsmtSSB1 causes elevated illegitimate recombinations in the mitochondrial genome, altered mitochondrial morphology, and compromised energy supply, suggesting that the OsmtSSB1-mediated mitochondrial function plays a critical role in subaleurone cell-fate determination in rice. |
| URI | http://hdl.handle.net/20.500.11897/623173 |
| ISSN | 1674-2052 |
| DOI | 10.1016/j.molp.2021.05.016 |
| Indexed | SCI(E) |
| Appears in Collections: | 现代农学院 |
