| Title | Clinical factors associated with composition of lung microbiota and important taxa predicting clinical prognosis in patients with severe community-acquired pneumonia |
| Authors | Du, Sisi Wu, Xiaojing Li, Binbin Wang, Yimin Shang, Lianhan Huang, Xu Xia, Yudi Yu, Donghao Lu, Naicong Liu, Zhibo Wang, Chunlei Liu, Xinmeng Xiong, Zhujia Zou, Xiaohui Lu, Binghuai Liu, Yingmei Zhan, Qingyuan Cao, Bin |
| Affiliation | Capital Med Univ, Clin Ctr Pulm Infect, Natl Ctr Resp Med, China Japan Friendship Hosp,Natl Clin Res Ctr Res, Beijing 100029, Peoples R China Chinese Acad Med Sci, Peking Union Med Coll, Inst Resp Med, Beijing 100029, Peoples R China China Japan Friendship Hosp, Ctr Resp Dis, Dept Pulm & Crit Care Med, Beijing 100029, Peoples R China Beijing Univ Chinese Med, Beijing 100029, Peoples R China Beijing Luhe Hosp, Beijing 101100, Peoples R China Tsinghua Univ, Peking Univ, Joint Ctr Life Sci, Beijing 100084, Peoples R China |
| Keywords | INJURY MANAGEMENT EXPRESSION MORTALITY |
| Issue Date | Jul-2021 |
| Publisher | FRONTIERS OF MEDICINE |
| Abstract | Few studies have described the key features and prognostic roles of lung microbiota in patients with severe community-acquired pneumonia (SCAP). We prospectively enrolled consecutive SCAP patients admitted to ICU. Bronchoscopy was performed at bedside within 48 h of ICU admission, and 16S rRNA gene sequencing was applied to the collected bronchoalveolar lavage fluid. The primary outcome was clinical improvements defined as a decrease of 2 categories and above on a 7-category ordinal scale within 14 days following bronchoscopy. Sixty-seven patients were included. Multivariable permutational multivariate analysis of variance found that positive bacteria lab test results had the strongest independent association with lung microbiota (R-2 = 0.033; P = 0.018), followed by acute kidney injury (AKI; R-2 = 0.032; P = 0.011) and plasma MIP-1 beta level (R-2 = 0.027; P = 0.044). Random forest identified that the families Prevotellaceae, Moraxellaceae, and Staphylococcaceae were the biomarkers related to the positive bacteria lab test results. Multivariable Cox regression showed that the increase in alpha-diversity and the abundance of the families Prevotellaceae and Actinomycetaceae were associated with clinical improvements. The positive bacteria lab test results, AKI, and plasma MIP-1 beta level were associated with patients' lung microbiota composition on ICU admission. The families Prevotellaceae and Actinomycetaceae on admission predicted clinical improvements. |
| URI | http://hdl.handle.net/20.500.11897/622934 |
| ISSN | 2095-0217 |
| DOI | 10.1007/s11684-021-0856-3 |
| Indexed | SCI(E) |
| Appears in Collections: | 生命科学学院 |
