| Title | NEK7 Promotes Pancreatic Cancer Progression And Its Expression Is Correlated With Poor Prognosis |
| Authors | Yan, Zilong Qu, Jianhua Li, Zhangfu Yi, Jing Su, Yanze Lin, Qirui Yu, Guangyin Lin, Zewei Yin, Weihua Lu, Fengmin Liu, Jikui |
| Affiliation | Shenzhen Peking Univ Hong Kong Univ Sci & Technol, Dept Hepatobiliary Surg, Peking Univ Shenzhen Hosp, Shenzhen, Peoples R China Peking Univ Shenzhen Hosp, Dept Hepatobiliary Surg, Shenzhen, Peoples R China Peking Univ Shenzhen Hosp, Dept Pathol, Shenzhen, Peoples R China Peking Univ Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, Beijing, Peoples R China Peking Univ Hlth Sci Ctr, Sch Basic Med Sci, Ctr Infect Dis, Beijing, Peoples R China |
| Keywords | PROTEIN-KINASES NIMA MITOSIS NEVER CARCINOMA FAMILY GENES |
| Issue Date | 6-Jul-2021 |
| Publisher | FRONTIERS IN ONCOLOGY |
| Abstract | The prognosis for pancreatic ductal adenocarcinoma (PDAC) patients is still dismal. Elucidation of associated genomic alteration may provide effective therapeutic strategies for PDAC treatment. NIMA-related protein kinase 7 is widely expressed in various tumors, including breast cancer, colorectal cancer and lung cancer, and promotes the proliferation of liver cancer cells in vitro and in vivo. We investigated the protein expression level of NEK7 in tumor tissues and adjacent normal tissues using immunohistochemistry of 90 patients with PADC. Meanwhile, the RNA expression level of NEK7 was examined using database-based bioinformatic analysis. Correlation and significance of NEK7 expression with patient clinicopathological features and prognosis were examined. Cell proliferation, cell adhesion, migration and invasion capabilities were measured following downregulation of NEK7 expression. 3D tumor organoids of pancreatic cancer were established and splenic xenografted into nude mice, then liver metastatic ability of NEK7 was evaluated in following 4 weeks. We observed NEK7 expression was upregulated in tumor tissues compared to normal tissues at both RNA and protein levels using bioinformatic analysis and immunohistochemistry analysis in PDAC. NEK7 expression was undetectable in normal pancreatic ducts; NEK7 was overexpressed in primary tumor of PDAC; NEK7 expression was highly correlated with advanced T stage, poorly differentiated histological grade invasive ductal carcinoma, and lymphatic invasion. Meanwhile, patients with higher NEK7 expression accompanied by worse survival outcome. Moreover, NEK7 promoted migration, invasion, adhesion, proliferation and liver metastatic ability of pancreatic cancer cells. Taken together, our data indicate that NEK7 promotes pancreatic cancer progression and it may be a potential marker for PDAC prognosis. |
| URI | http://hdl.handle.net/20.500.11897/622854 |
| ISSN | 2234-943X |
| DOI | 10.3389/fonc.2021.705797 |
| Indexed | SCI(E) |
| Appears in Collections: | 深圳医院 |
