| Title | Mesenchymal stem cells loaded on 3D-printed gradient poly(epsilon-caprolactonelimethacrylated alginate composite scaffolds for cartilage tissue engineering |
| Authors | Cao, Yanyan Cheng, Peng Sang, Shengbo Xiang, Chuan An, Yang Wei, Xiaochun Shen, Zhizhong Zhang, Yixia Li, Pengcui |
| Affiliation | Taiyuan Univ Technol, Coll Informat & Comp, MicroNano Syst Res Ctr,Minist Educ, Key Lab Adv Transducers & Intelligent Control Sys, Taiyuan 030024, Peoples R China Hebei North Univ, Coll Informat Sci & Engn, Zhangjiakou 075000, Peoples R China Shanxi Med Univ, Dept Orthoped, Shanxi Key Lab Bone & Soft Tissue Injury Repair, Hosp 2, Taiyuan 030001, Peoples R China Peking Univ Third Hosp, Dept Plast Surg, Beijing 100191, Peoples R China Taiyuan Univ Technol, Coll Biomed Engn, Dept Biomed Engn, Taiyuan 030024, Peoples R China |
| Keywords | 3D PRINTED SCAFFOLDS ARTICULAR-CARTILAGE PORE-SIZE COMPRESSIVE MODULUS ZONAL ORGANIZATION HYDROGEL MICROSPHERES CHONDROCYTES |
| Issue Date | Jun-2021 |
| Publisher | REGENERATIVE BIOMATERIALS |
| Abstract | Cartilage has limited self-repair ability due to its avascular, alymphatic and aneural features. The combination of three-dimensional (3D) printing and tissue engineering provides an up-and-coming approach to address this issue. Here, we designed and fabricated a tri-layered (superficial layer (SL), middle layer (ML) and deep layer (DL)) stratified scaffold, inspired by the architecture of collagen fibers in native cartilage tissue. The scaffold was composed of 3D printed depth-dependent gradient poly(epsilon-caprolactone) (PCL) impregnated with methacrylated alginate (ALMA), and its morphological analysis and mechanical properties were tested. To prove the feasibility of the composite scaffolds for cartilage regeneration, the viability, proliferation, collagen deposition and chondrogenic differentiation of embedded rat bone marrow mesenchymal stem cells (BMSCs) in the scaffolds were assessed by Live/dead assay, CCK-8, DNA content, cell morphology, immunofluorescence and real-time reverse transcription polymerase chain reaction. BMSCs-loaded gradient PCL/ALMA scaffolds showed excellent cell survival, cell proliferation, cell morphology, collagen II deposition and hopeful chondrogenic differentiation compared with three individual-layer scaffolds. Hence, our study demonstrates the potential use of the gradient PCL/ALMA construct for enhanced cartilage tissue engineering. |
| URI | http://hdl.handle.net/20.500.11897/622794 |
| ISSN | 2056-3418 |
| DOI | 10.1093/rb/rbab019 |
| Indexed | SCI(E) |
| Appears in Collections: | 第三医院 |
