| Title | MicroRNA-210-3p Promotes Chondrogenic Differentiation and Inhibits Adipogenic Differentiation Correlated with HIF-3 alpha Signalling in Bone Marrow Mesenchymal Stem Cells |
| Authors | Yang, Meng Yan, Xin Yuan, Fu-Zhen Ye, Jing Du, Ming-Ze Mao, Zi-Mu Xu, Bing-Bing Chen, You-Rong Song, Yi-Fan Fan, Bao-Shi Yu, Jia-Kuo |
| Affiliation | Peking Univ Third Hosp, Sports Med Dept, Beijing Key Lab Sports Injuries, Beijing, Peoples R China Peking Univ, Inst Sports Med, Beijing, Peoples R China Weifang Med Univ, Sch Clin Med, Weifang, Peoples R China |
| Keywords | ARTICULAR-CARTILAGE CANCER |
| Issue Date | 12-Apr-2021 |
| Publisher | BIOMED RESEARCH INTERNATIONAL |
| Abstract | Cartilage injury of the knee joint is very common. Due to the limited self-healing ability of articular cartilage, osteoarthritis is very likely to occur if left untreated. Bone marrow mesenchymal stem cells (BMMSCs) are widely used in the study of cartilage injury due to their low immunity and good amplification ability, but they still have disadvantages, such as heterogeneous undifferentiated cells. MicroRNAs can regulate the chondrogenic differentiation ability of MSCs by inhibiting or promoting mRNA translation and degradation. In this research, we primarily investigated the effect of microRNA-210-3p (miR-210-3p) on chondrogenic and adipogenic differentiation of BMMSCs in vitro. Our results demonstrate that miR-210-3p promoted chondrogenic differentiation and inhibited adipogenic differentiation of rat BMMSCs, which was related to the HIF-3 alpha signalling pathway. Additionally, miR-210-3p promotes mRNA and protein levels of the chondrogenic expression genes COLII and SOX9 and inhibits mRNA and protein levels of the adipogenic expression genes PPAR gamma and LPL. Thus, miR-210-3p combined with BMMSCs is a candidate for future clinical applications in cartilage regeneration and could represent a promising new therapeutic target for OA. |
| URI | http://hdl.handle.net/20.500.11897/619321 |
| ISSN | 2314-6133 |
| DOI | 10.1155/2021/6699910 |
| Indexed | SCI(E) |
| Appears in Collections: | 第三医院 |
