| Title | 3D-printed cell-free PCL-MECM scaffold with biomimetic micro-structure and micro-environment to enhance in situ meniscus regeneration |
| Authors | Guo, Weimin Chen, Mingxue Wang, Zhenyong Tian, Yue Zheng, Jinxuan Gao, Shuang Li, Yangyang Zheng, Yufeng Li, Xu Huang, Jingxiang Niu, Wei Jiang, Shuangpeng Hao, Chunxiang Yuan, Zhiguo Zhang, Yu Wang, Mingjie Wang, Zehao Peng, Jiang Wang, Aiyuan Wang, Yu Sui, Xiang Xu, Wenjing Hao, Libo Zheng, Xifu Liu, Shuyun Guo, Quanyi |
| Affiliation | Sun Yat Sen Univ, Affiliated Hosp 1, Dept Orthopaed Surg, 58 Zhongshan Second Rd, Guangzhou 510080, Guangdong, Peoples R China Chinese Peoples Liberat Army Gen Hosp, Med Ctr 1, Inst Orthoped, 28 Fuxing Rd, Beijing 100853, Peoples R China Beijing Key Lab Regenerat Med Orthoped, Key Lab Musculoskeletal Trauma & War Injuries PLA, 28 Fuxing Rd, Beijing 100853, Peoples R China Peking Univ, Clin Coll 4, Beijing Jishuitan Hosp, Dept Orthoped Surg, 31 Xinjiekou East St, Beijing 100035, Peoples R China Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guanghua Sch Stomatol, Dept Orthodont, 56 Linyuan Xi Rd, Guangzhou 510055, Guangdong, Peoples R China Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomat & Tissue Engn, Beijing 100871, Peoples R China Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Innovat Orthopaed Biomat & Drug Translat Res Lab, Musculoskeletal Res Lab,Dept Orthopaed & Traumato, Hong Kong, Peoples R China Chinese Peoples Liberat Army Gen Hosp, Med Ctr 1, Inst Anesthesiol, 28 Fuxing Rd, Beijing 100853, Peoples R China Dalian Med Univ, Affiliated Hosp 1, Dept Orthoped Surg, 222 Zhongshan Rd, Dalian 116011, Peoples R China |
| Keywords | MESENCHYMAL STEM-CELLS EXTRACELLULAR-MATRIX TENSILE PROPERTIES REPAIR KNEE VITRO COLLAGEN SCORE |
| Issue Date | Oct-2021 |
| Publisher | BIOACTIVE MATERIALS |
| Abstract | Despite intensive effort was made to regenerate injured meniscus by cell-free strategies through recruiting endogenous stem/progenitor cells, meniscus regeneration remains a great challenge in clinic. In this study, we found decellularized meniscal extracellular matrix (MECM) preserved native meniscal collagen and glycosami-noglycans which could be a good endogenous regeneration guider for stem cells. Moreover, MECM significantly promoted meniscal fibrochondrocytes viability and proliferation, increased the expression of type II collagen and proteoglycans in vitro. Meanwhile, we designed 3D-printed polycaprolactone (PCL) scaffolds which mimic the circumferential and radial collagen orientation in native meniscus. Taken these two advantages together, a micro-structure and micro-environment dually biomimetic cell-free scaffold was manipulated. This cell-free PCL-MECM scaffold displayed superior biocompatibility and yielded favorable biomechanical capacities closely to native meniscus. Strikingly, neo-menisci were regenerated within PCL-MECM scaffolds which were transplanted into knee joints underwent medial meniscectomy in rabbits and sheep models. Histological staining confirmed neo-menisci showed meniscus-like heterogeneous staining. Mankin scores showed PCL-MECM scaffold could protect articular cartilage well, and knee X-ray examination revealed same results. Knee magnetic resonance imaging (MRI) scanning also showed some neo-menisci in PCL-MECM scaffold group. In conclusion, PCL-MECM scaffold appears to optimize meniscus regeneration. This could represent a promising approach worthy of further investigation in preclinical applications. |
| URI | http://hdl.handle.net/20.500.11897/618985 |
| DOI | 10.1016/j.bioactmat.2021.02.019 |
| Indexed | SCI(E) |
| Appears in Collections: | 北京积水潭医院 前沿交叉学科研究院 工学院 |
