| Title | Pharmacokinetics study of 16 active ingredients from Tabson-2 decoction in normal andd-galactose induced osteoporosis rats by liquid chromatography-tandem mass spectrometry |
| Authors | Li, Na Dong, Xin Ma, Feixiang Tian, Xianting Bu, Ren Li, Chunyan Gao, Peng Lu, Yingyuan Xue, Peifeng Guo, Xiaoyu Tu, Pengfei |
| Affiliation | Inner Mongolia Med Univ, Dept Pharm, Hohhot, Peoples R China Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing, Peoples R China |
| Keywords | BIOAVAILABILITY QUANTIFICATION PLASMA MS/MS ACID |
| Issue Date | Aug-2020 |
| Publisher | JOURNAL OF SEPARATION SCIENCE |
| Abstract | Tabson-2 decoction is the traditional Mongolian formula for anti-osteoporosis, and the ambiguous of active ingredient is an important factor in restricting its modernization and globalization. Although pharmacokinetic profiles research is a viable approach to find the components being responsible for formula efficacy, the pharmacokinetics study of Tabson-2 decoction has not been elucidated yet. Owing to the existence of isomers, low bioavailability of some small molecule and interference of endogenous, the pharmacokinetics study of Tabson-2 decoction are more difficult than that of chemical drugs. In our experiment, a specific and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination of 16 active ingredients in Tabson-2 decoction, which could fulfill the requirements of multi-compounds pharmacokinetic study of Tabson-2 decoction. Additionally, the ingredients with significant distributions in rats were gentianic acid, chlorogenic acid, and aucubin, which could be the main potential active components in Tabson-2 decoction. The components with a significant bioavailability difference between normal andd-galactose induced osteoporosis rats were achieved as well. These data offer useful information for screening the active ingredients in Tabson-2 decoction, and assessing the bioavailability of these active ingredients in different physiological status, which might provide a possible mechanism of anti-osteoporosis efficacy of Tabson-2 decoction. |
| URI | http://hdl.handle.net/20.500.11897/607455 |
| ISSN | 1615-9306 |
| DOI | 10.1002/jssc.202000355 |
| Indexed | SCI(E) |
| Appears in Collections: | 药学院 天然药物与仿生药物国家重点实验室 |
