| Title | Aberrant spliceosome expression and altered alternative splicing events correlate with maturation deficiency in human oocytes |
| Authors | Li, Junsheng Lu, Minzhen Zhang, Puyao Hou, Entai Li, Tianjie Liu, Xian Xu, Xiaofei Wang, Zhaohui Fan, Yong Zhen, Xiumei Li, Rong Liu, Ping Yu, Yang Hang, Jing Qiao, Jie |
| Affiliation | Peking Univ Third Hosp, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing, Peoples R China Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing, Peoples R China Guangzhou Med Univ, Key Lab Major Obstet Dis Guangdong Prov, Affiliated Hosp 3, Guangzhou, Peoples R China Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China |
| Keywords | RNA MUTATIONS TRANSCRIPTOME STIMULATION PROTEINS SEQUENCE PATHWAY EMBRYOS IVF |
| Issue Date | Aug-2020 |
| Publisher | CELL CYCLE |
| Abstract | Different strategies of ovarian stimulation are widely used in IVF to retrieve mature metaphase II (MII) oocytes for fertilization. On average, approximately 70% of recovered oocytes are mature, while personalized administration of hCG and/or GnRH agonist trigger andin vitromaturation (IVM) management can further improve the maturation rate. However, even under such conditions, a complete absence of oocyte maturation is still observed sporadically. The probable causes for such maturation-deficient (MD) oocytes - which arrest abnormally at metaphase I (MI) stage - are still under investigation. In the present study, using single-cell transcriptomic RNA sequencing (RNA-seq) and differential expression analysis, we showed that gene expression profiles were aberrant, and alternative splicing (AS) patterns were changed in MD oocytes when compared with normally mature (MN) oocytes. Gene ontology (GO) enrichment demonstrated that the differently expressed genes (DEGs) were mostly correlated with pre-mRNA splicing, RNA transportation, RNA processing, and mRNA regulation. Subsequently, analysis of AS events revealed that genes with altered AS patterns were primarily associated with metabolism and cell cycle. With these findings, we have demonstrated aberrant gene expression in complete maturation-deficient oocytes, and we propose that alterations in post-transcriptional regulation constitute a potential underlying mechanism governing oocyte maturation. |
| URI | http://hdl.handle.net/20.500.11897/607390 |
| ISSN | 1538-4101 |
| DOI | 10.1080/15384101.2020.1799295 |
| Indexed | SCI(E) |
| Appears in Collections: | 第三医院 生命科学学院 |
