| Title | Dual-Controlled Release of Icariin/Mg2+ from Biodegradable Microspheres and Their Synergistic Upregulation Effect on Bone Regeneration |
| Authors | Yuan, Zuoying Wan, Zhuo Wei, Pengfei Lu, Xin Mao, Jianping Cai, Qing Zhang, Xu Yang, Xiaoping |
| Affiliation | Beijing Univ Chem Technol, State Key Lab Organ Inorgan Composites, Beijing 100029, Peoples R China Beijing Univ Chem Technol, Beijing Lab Biomed Mat, Beijing 100029, Peoples R China Peking Univ, Dept Mech & Engn Sci, Coll Engn, Beijing 100871, Peoples R China Tianjin Med Univ, Sch & Hosp Stomatol, Dept Endodont, Tianjin 300070, Peoples R China Beijing Jishuitan Hosp, Dept Spine Surg, Beijing 100035, Peoples R China |
| Keywords | OSTEOGENIC DIFFERENTIATION STEM-CELLS NANOFIBROUS MICROSPHERES SCAFFOLDS DELIVERY FLAVONOIDS MEMBRANE IMPROVE BMP-2 |
| Issue Date | Apr-2020 |
| Publisher | ADVANCED HEALTHCARE MATERIALS |
| Abstract | Current scaffolds applied for bone tissue engineering are still lacking sufficient osteogenic capacity to induce efficient bone regeneration. Biodegradable microsphere-type scaffolds are designed to achieve the dual-controlled release of a Chinese medicine (i.e., icariin, ICA) and a bioactive ion (i.e., Mg2+), in order to achieve their synergistic effect on inducing osteogenesis. The hydrophobic icariin is preloaded onto MgO/MgCO3 (1:1 in weight ratio) particles at different amounts and then the particles are encapsulated into biodegradable poly(lactide-co-glycolide) (PLGA) microspheres (PMI) at a fixed fraction (20 wt%). Continuous releases of Mg2+ ion and icariin from the microspheres are detected, showing dependence on icariin amounts. At an optimized moderate loading amount, the resulting PMI-M microspheres display the strongest activation effect on cell biological behaviors among all the designs. By implanting the PMI-M microspheres into rat calvarial defects for 16 weeks, it is found that they can effectively enhance new bone formation, presenting significantly higher capacity in inducing osteogenesis than PMg (containing MgO/MgCO3 but without icariin) and blank PLGA microspheres. Clearly, the released Mg2+ ions are beneficial to osteogenesis, and the coincorporation of icariin exerts supplemental effects in inducing new bone formation, which suggest a promising strategy to regenerate severe bone injuries by designing a dual-release system. |
| URI | http://hdl.handle.net/20.500.11897/606726 |
| ISSN | 2192-2640 |
| DOI | 10.1002/adhm.202000211 |
| Indexed | SCI(E) Scopus EI |
| Appears in Collections: | 工学院 |
