| Title | Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging |
| Authors | Zhang, Yulin Wang, Jianyong Xing, Shijia Li, Liuju Zhao, Shiqun Zhu, Wenzhen Liang, Kuo Liu, Yanmei Chen, Liangyi |
| Affiliation | Peking Univ, Inst Mol Med, State Key Lab Membrane Biol, Beijing Key Lab Cardiometab Mol Med, Beijing 100871, Peoples R China PKU IDG McGovern Inst Brain Res, Beijing 100871, Peoples R China Capital Med Univ, Xuanwu Hosp, Dept Gen Surg, Beijing 100053, Peoples R China South China Normal Univ, Inst Brain Res & Rehabil, Guangdong Key Lab Mental Hlth & Cognit Sci, Guangzhou 200062, Peoples R China |
| Keywords | ENDOPLASMIC-RETICULUM CA2+ MICRODOMAINS ORGANIZATION DOMAINS |
| Issue Date | Apr-2020 |
| Publisher | SCIENCE CHINA-LIFE SCIENCES |
| Abstract | Despite the wide application of super-resolution (SR) microscopy in biological studies of cells, the technology is rarely used to monitor functional changes in live cells. By combining fast spinning disc-confocal structured illumination microscopy (SD-SIM) with loading of cytosolic fluorescent Ca2+ indicators, we have developed an SR method for visualization of regional Ca2+ dynamics and related cellular organelle morphology and dynamics, termed SR calcium lantern imaging. In COS-7 cells stimulated with ATP, we have identified various calcium macrodomains characterized by different types of Ca-2+ release from endoplasmic reticulum (ER) stores. Finally, we demonstrated various roles of mitochondria in mediating calcium signals from different sources; while mitochondria can globally potentiate the Ca-2+ entry associated with store release, mitochondria also locally control Ca2+ release from the neighboring ER stores and assist in their refilling processes. |
| URI | http://hdl.handle.net/20.500.11897/606637 |
| ISSN | 1674-7305 |
| DOI | 10.1007/s11427-019-1659-4 |
| Indexed | SCI(E) Scopus |
| Appears in Collections: | 分子医学研究所 膜生物学国家重点实验室 |
