| Title | Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions |
| Authors | Liu, Xianmei Xia, Xue Wang, Xifu Zhou, Jing Sung, Lanping Amy Long, Jinhua Geng, Xueyu Zeng, Zhu Yao, Weijuan |
| Affiliation | Guizhou Med Univ, Sch Basic Med Sci, Sch Biol & Engn, Guiyang, Peoples R China Peking Univ, Hemorheol Ctr, Sch Basic Med Sci, Dept Physiol & Pathophysiol,Hlth Sci Ctr, Beijing, Peoples R China Capital Med Univ, Beijing Anzhen Hosp, Dept Emergency, Beijing, Peoples R China Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Hlth Ctr, Beijing, Peoples R China |
| Issue Date | 15-Jan-2021 |
| Publisher | FRONTIERS IN IMMUNOLOGY |
| Abstract | Dendritic cells (DCs) are the most potent antigen-presenting cells. Upon maturation, DCs express costimulatory molecules and migrate to the lymph nodes to present antigens to T cells. The actin cytoskeleton plays key roles in multiple aspects of DC functions. However, little is known about the mechanisms and identities of actin-binding proteins that control DC maturation and maturation-associated functional changes. Tropomodulin1 (Tmod1), an actin-capping protein, controls actin depolymerization and nucleation. We found that Tmod1 was expressed in bone marrow-derived immature DCs and was significantly upregulated upon lipopolysaccharide (LPS)-induced DC maturation. By characterizing LPS-induced mature DCs (mDCs) from Tmod1 knockout mice, we found that compared with Tmod1(+/+) mDCs, Tmod1-deficient mDCs exhibited lower surface expression of costimulatory molecules and chemokine receptors and reduced secretion of inflammatory cytokines, suggesting that Tmod1 deficiency retarded DC maturation. Tmod1-deficient mDCs also showed impaired random and chemotactic migration, deteriorated T-cell stimulatory ability, and reduced F-actin content and cell stiffness. Furthermore, Tmod1-deficient mDCs secreted high levels of IFN-beta and IL-10 and induced immune tolerance in an experimental autoimmune encephalomyelitis (EAE) mouse model. Mechanistically, Tmod1 deficiency affected TLR4 signaling transduction, resulting in the decreased activity of MyD88-dependent NF kappa B and MAPK pathways but the increased activity of the TRIF/IRF3 pathway. Rescue with exogenous Tmod1 reversed the effect of Tmod1 deficiency on TLR4 signaling. Therefore, Tmod1 is critical in regulating DC maturation and immune functions by regulating TLR4 signaling and the actin cytoskeleton. Tmod1 may be a potential target for modulating DC functions, a strategy that would be beneficial for immunotherapy for several diseases. |
| URI | http://hdl.handle.net/20.500.11897/604637 |
| ISSN | 1664-3224 |
| DOI | 10.3389/fimmu.2020.587441 |
| Indexed | SCI(E) |
| Appears in Collections: | 基础医学院 |
